Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Extractive Spectro Estimation of Clarithromycin and Clindamycin in Bulk and Dosage Forms


Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
     

   Subscribe/Renew Journal


Two spectrophotometric methods are described for determination of Clarithromycin and Clindamycin in bulk and pharmaceutical dosage forms using two acidic dyes, bromocresol purple and Bromocresol green to form ion pair extractable complexes with clarithromycin and clindamycin HCl then measuring absorbances at (390nm for clarithromycin or 397 nm for clindamycin) and 413 nm respectively. The effect,s of acidity, buffer volume and dye concentration, on the absorption were studied. Calibration curves were linear over ranges of 12-28 μg.ml-1 for Clarithromycin and 8- 40 μg.ml-1 for Clindamycin in case of bromocresol purple and of 4-20 μg.ml-1 for Clarithromycin and 16-32μg.ml-1 for Clindamycin in case of Bromocresol green. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords

Clarithromycin, Clindamycin, Bromocresol Purple and Bromocresol Green.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 50

PDF Views: 0




  • Extractive Spectro Estimation of Clarithromycin and Clindamycin in Bulk and Dosage Forms

Abstract Views: 50  |  PDF Views: 0

Authors

Sobhy M. El-Adl
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Mohamed El. Hossinny El. Sadek
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt
Marwa Hamdy Hassan
Department of Medicinal Chemistry, Zagazige University, Zagazig, Egypt

Abstract


Two spectrophotometric methods are described for determination of Clarithromycin and Clindamycin in bulk and pharmaceutical dosage forms using two acidic dyes, bromocresol purple and Bromocresol green to form ion pair extractable complexes with clarithromycin and clindamycin HCl then measuring absorbances at (390nm for clarithromycin or 397 nm for clindamycin) and 413 nm respectively. The effect,s of acidity, buffer volume and dye concentration, on the absorption were studied. Calibration curves were linear over ranges of 12-28 μg.ml-1 for Clarithromycin and 8- 40 μg.ml-1 for Clindamycin in case of bromocresol purple and of 4-20 μg.ml-1 for Clarithromycin and 16-32μg.ml-1 for Clindamycin in case of Bromocresol green. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical dosage forms and results were compared statistically with reference methods.

Keywords


Clarithromycin, Clindamycin, Bromocresol Purple and Bromocresol Green.