Asian Journal of Research in Chemistry

G. Venkata Prasad ¹, S. Sravani ¹, B. Mohammed Ishaq ¹, M. Madhu ¹, Sreenivasulu Munna ¹, C. Gopinath ¹

Affiliations
1 Department of Pharmaceutical Analysis, Annamacharya college of Pharmacy, Rajampet, Kadapa Dist, A.P, IN

Abstract

To develop simple, sensitive, selective and accurate UV Spectrophotometric methods for the determination of Cephalexin in bulk drug and pharmaceutical formulations (Capsules). The absorbances were measured at 261(Method A) and 257 nm (Method B). The methods was found to be linear from a quantitation ranges of 5μg/ml to 40μg/ml (Method A) in Phosphate Buffer pH 2.0 and 5μg/ml to 50μg/ml (Method B) in 0.1 N HCl. The regression of the curves was Y = 0.020x - 0.021 (Method A) and Y = 0.014x - 0.102 (Method B). The methods gave satisfactory results in terms of repeatability and intermediate precision (RSD<1.010%) (Method A) and (RSD<0.589%) (Method B) 0.855 μg/mL and 2.85 μg/mL (Method A) and 2.357 μg/mL and 7.857 μg/mL were the LOD and LOQ values, respectively. The methods was validated and proved to be robust and rugged. The results of analysis for these methods have been validated statistically and by recovery studies.

Keywords

Cephalexin, Spectroscopic Method, Phosphate Buffer (Ph 2.0), 0.1 N Hcl

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G. Prashanthi ¹, Y. Pradeep Kumar ², D. Swarna Latha ³, N. Pramod ², C. Gopinath ²

Affiliations
1 Dept. of Pharmaceutical Chemistry, QIS College of Pharmacy, Vengamukka Palem, Ongole, 523272, IN
2 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, Rajampet, 516126, IN
3 Dept. of Pharmacognosy, Annamacharya College of Pharmacy, Rajampet, 516126, IN

Abstract

Novel Tetra hydro imidazo[1,2-a] pyrazine derivatives have been synthesized by Treatment of pyrazine-2- carboxylic acid with thionyl chloride in methanol yielded methyl pyrazine-2-carboxylate.This up on treatment with alcoholic ammonia gave pyrazinamide, further it was undergone Hoffman's degradation, gave 2-amino pyrazine after that it was refluxed for 26 hrs with chloro acetaldehyde , sodium bicarbonate gave imidazo[1,2-a] pyrazine and hydrogen gas was passed on it in the presence of palladium on carbon yielded tetra hydro imidazo[1,2-a] pyrazine ,which was treated with different substituent's to get the novel derivatives. Synthesized compounds were identified and characterized by Melting point, TLC, FT-IR, ¹H and ¹³C NMR , MASS and percent purity done by HPLC . Based on characterization and percent purity novel derivatives were subjected for molecular docking , QSAR studies and pharmacological evaluation. Selected compounds were docked on Antioxidant activity using "Thioredoxin peroxidase B" as a target site. QSAR parameters like partition coefficient was studied and showed polar surface area below 140 A⁰ and passed ADME parameters. Analysis of docking and QSAR studies suggested that remarkable inhibitory activity was exhibited by the derivatives. Hydrogen bond interactions were mapped to confirm their potencies. They were evaluated for In-Vitro Anti-oxidant activity by DPPH method and In-Vitro Anti-diabetic activity by alpha-amylase inhibition assay by DNSA method. Results suggested that electron withdrawing groups like nitro, bromo having compounds shown potent activity. Rest of compounds showed mild to moderate activity.

Keywords

Tetra Hydro Imidazo[1,2-A] Pyrazine, Docking and Q SAR, Anti-Oxidant, Anti-Diabetic Activities.

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Y. Pradeep Kumar ¹, S. Chand Basha ¹, K. Anusha ², K. Madhuri ², C. Gopinath ¹

Affiliations
1 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Kadapa (Dist) A.P, 516115, IN
2 Dept. of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Kadapa (Dist) A.P, 516115, IN

Abstract

Non classical, High-speed, environmentally benign synthesis with microwaves has attracted researchers for organic synthesis was a considerable amount of attention in recent years. An expeditious one pot microwave irradiation method for preparation of 2- azetidinones is developed. This method has been assessed as greener methodology. In our present study, A series of six novel 2-azetidinones were synthesized, compounds were identified by melting point and thin layer chromatography , functional groups of synthesized compounds were confirmed by IR spectroscopy, compounds were evaluated for their antimicrobial activities. The activities were due to cyclic carbonyl group in 2-azetidinones. Some of the compounds have shown comparative antimicrobial activities against all the microbial strains.

Keywords

Microwave Synthesis, 2-Azetedinone, Anti Bacterial Activity.

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