Open Access Subscription Access
Open Access Subscription Access
RP-HPLC Method for the Simultaneous Estimation of Moxifloxacin Hydrochloride and Cefixime Trihydrate in Synthetic Mixture
A simple, selective, accurate Reverse Phase High Performance Liquid Chromatographic method was developed and validated for the simultaneous estimation of Moxifloxacin hydrochloride and Cefixime trihydrate in their synthetic mixture. Chromatographic separation achieved isocratically on a thermoscientific BDS Hypersil C18 (250mm X 4.6 mm i.d., 5 μm particle size) column utilizing a mobile phase of acetonitrile: tetrabutyl ammonium hydrogen sulphate (TBAHS) + tetrabutyl ammonium bromide (TBAB) buffer pH 3 (20:80 v/v) and adjusting pH 3 with orthophosphoric acid at a flow rate of 1.5ml/min with UV detection at 280.8nm. The retention time for Moxifloxacin hydrochloride and Cefixime trihydrate was found to be 3.078 and 5.263 min respectively. Linearity was obtained in the concentration range of 10-60μg/ml of Moxifloxacin hydrochloride and 10-60μg/ml of Cefixime trihydrate with a correlation coefficient of 0.999 and 0.999 respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation. This study aimed at developing and validating an HPLC method being simple, accurate, robust and selective and the proposed method can be used for the estimation of these drugs in combined synthetic mixture.
Simultaneous Estimation, RP-HPLC, Moxifloxacin Hydrochloride, Cefixime Trihydrate
- Skoog DA., West DM., and Hollar FJ. Fundamentals of Analytical Chemistry; 8th Edn; Saunders College Publishing, Philadelphia, 2004, pp 973-992.
- Beckett AH., and Stenlake JB. UV-visible Spectrophotometry: Practical Pharmaceutical Chemistry Part-II; 4th Edn; CBS Publishers and Distributors, New Delhi, 2001, pp 285-97.
- Sethi PD. High Performance Liquid Chromatography- Quantitative Analysis of Pharmaceutical Formulations; reprint; CBS Publishers and Distributors, New Delhi, 2008, pp 14-15, 116-120.
- ICH Q2B Guideline, Validation of Analytical Procedures: Methodology, ICH Harmonized Tripartite Guidelines, 1996.
- ICH Q2A Guideline, Validation of Analytical Procedures: Text and Methodology, ICH Harmonized Tripartite Guideline, 1995. 6. Potdar MA., Current Good Manufacturing Practices for Pharmaceuticals, 1st edition, Pharmamed Press, Hyderabad, 2009, pp 462-468.
- Baertschi SW., and Raynold DW. Pharmaceutical Stress Testing- Predicting drug Degradation; special Indian Edn; Eli Lilly and Company, Indianapolis, 2005(reprint-2008), pp 2-4, 13,14.
- “Drug profile of cefixime” www.drugbank.ca/cefixime
- “Drug profile of cefixime” http://www.drugs.com/cdi/ cefixime.html
- British Pharmacopoeia; Volume I; British Pharmacopoeia commission; London: HMSO Publication, 2009, pp 1139-1142.
- The Merck Index; 14th Edn; Merck Research Laboratories, 2006, pp 315.
- “Drug profile of moxifloxacin” www.drugbank.ca/moxifloxacin
- “Drug profile of moxifloxacin” http://www.drugs.com/mtm/ moxifloxacin.html
- British Pharmacopoeia; Volume I; British Pharmacopoeia commission; London: HMSO Publication, 2009, pp 4052-4053.
- The Merck Index; 14th Edn; Merck Research Laboratories, 2006, pp 1087.
- United States Pharmacopeia-35 and National Formulary-30; Volume II; The United States Pharmacopoeia Convention Inc., 2009, pp 1654.
- British Pharmacopoeia; Volume I; British Pharmacopoeia Commission; London: HMSO Publication, 2009, pp 1140-1141.
- Europian Pharmacopeia 5.0; The European Pharmacopoeia Commission; The European Directorate for the quality of Medicines (EDQM), 2005, pp 1212.
- Khandagle KS, Gandhi SV, Deshpande PB and Gaikwad NV, “A simple and sensitive RP-HPLC method for simultaneous estimation of cefixime and ofloxacin in combined tablet dosage form.” Int. J. Pharm. Pharm. Sci. 2011, 3, 46-48.
- Deshpande MM, Kasture VS and Gosavib SA, “Application of HPLC and HPTLC for the Simultaneous Determination of Cefixime Trihydrate and Ambroxol Hydrochloride in Pharmaceutical Dosage Form.” Eurasian J. Anal. Chem. 2010, 5, 227-238.
- Shah PB and Pundarikakshudu K, “Spectrophotometric, Difference Spectroscopic, and High-Performance Liquid Chromatographic Methods For the determination of Cefixime in pharmaceutical formulations.” J. AOAC. Int. 2006, 89, 987-994.
- United States Pharmacopeia-35 and National Formulary-30; Volume II; The United States Pharmacopoeia Convention Inc., 2009, pp 3959.
- British Pharmacopoeia; Volume I; British Pharmacopoeia Commission; London: HMSO Publication, 2009, pp 4053-4054.
- Dewani AP, Barik BB, Kanungo SK, Wattyani BR and Chandewar AV, “Development and Validation of RP-HPLC Method for the Determination of Moxifloxacin in Presence of Its Degradation Products.” Am.-Eurasian J. Sci. Res. 2011, 6, 192- 200.
- A.K. Hemanth Kumar and Geetha Ramachandran, “Simple and rapid liquid chromatography method for determination of moxifloxacin in plasma.” J. Chromatogr., B. 2009, 877, 1205- 1208.
- Motwani SK, Chopra S, Ahmad FJ and Khar RK, “Validated spectrophotometric methods for the estimation of moxifloxacin in bulk and pharmaceutical formulations.” Spectrochim. Acta, Part A. 2007, 68, 250-256.
- Motwani SK, Khar RK Ahmad FJ, Chopra S, Kohli K and Talegaonkar S, “Application of a validated stability-indicating densitometric thin layer chromatographic method to stress degradation studies on moxifloxacin.” Anal. Chim. Acta. 2007, 582, 75–82.
- Shah CK, Umalkar DK and Dr. Rajesh KS, “Simultaneous Spectrophotometric Determination of Cefixime and Moxifloxacin in Bulk Drug and Drug Formulation by Absorption Ratio Method”, 2012, http://www.pharmatutor.org/articles/simultaneousspectrophotomeric- determination-cefixime-moxifloxacin-bulkdrug- formulation-absorption-ratio-method
- Patel RK, Parmar RR, Patel VM and Shah DA, “Method Devlopment and Validation of Cefixime and Moxifloxacin in Pharmaceutical Dosage Form by UV Spectrophotometric Method.” Int. J. Pharm. Res. Bio-Sci. 2012, 1, 81-93.
- Pavia DL., Lampman GM., Kriz GS and Vyvyan JR. Spectroscopy; 11th Edn; Cengage Learning India Private Limited, New Delhi, reprint 2011, pp 35-39.
Abstract Views: 598
PDF Views: 2