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Evaluation of Residues of Chlorpyrifos and its Metabolite in Green Apples


Affiliations
1 Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India
2 Department of Organic Chemistry, Gayathri PG College, Vizianagaram, Andhra Pradesh, India
     

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A simple and inexpensive method was developed using solid-phase extraction, together with gas chromatographic method and high performance liquid chromatographic method for determination of chlorpyrifos and its metabolite O,O-diethyl triphosphate residues in green apples. The evaluated parameters include the extracts by silica gel packed column using methanol and water solvent mixture (90:10) and ethyl acetate solvents. The method was validated using green apple fruit samples spiked with chlorpyrifos and its metabolite (O,O-diethyl triphosphate) at different fortification levels (0.01 and 0.1 mg/kg). Average recoveries (using each concentration six replicates) of range 86-95%. The calibration solutions concentration in the range 0.01-5.0 mg/L and limit of detection (LOD) and limit of quantification (LOQ) were 0.003 mg/kg and 0.01 mg/kg respectively. Finally the green apple fruit residue samples were analyzed by GC and HPLC.

Keywords

HPLC, GC, Silica Gel, Chlorpyrifos, O,O-Diethyl Triphosphate and Green Apples.
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  • Evaluation of Residues of Chlorpyrifos and its Metabolite in Green Apples

Abstract Views: 53  |  PDF Views: 5

Authors

Tentu Nageswara Rao
Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India
Botsa Parvatamma
Department of Organic Chemistry, Gayathri PG College, Vizianagaram, Andhra Pradesh, India
Karri Apparao
Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India
Jakka Mastan
Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India

Abstract


A simple and inexpensive method was developed using solid-phase extraction, together with gas chromatographic method and high performance liquid chromatographic method for determination of chlorpyrifos and its metabolite O,O-diethyl triphosphate residues in green apples. The evaluated parameters include the extracts by silica gel packed column using methanol and water solvent mixture (90:10) and ethyl acetate solvents. The method was validated using green apple fruit samples spiked with chlorpyrifos and its metabolite (O,O-diethyl triphosphate) at different fortification levels (0.01 and 0.1 mg/kg). Average recoveries (using each concentration six replicates) of range 86-95%. The calibration solutions concentration in the range 0.01-5.0 mg/L and limit of detection (LOD) and limit of quantification (LOQ) were 0.003 mg/kg and 0.01 mg/kg respectively. Finally the green apple fruit residue samples were analyzed by GC and HPLC.

Keywords


HPLC, GC, Silica Gel, Chlorpyrifos, O,O-Diethyl Triphosphate and Green Apples.