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Preconcentration of Imidacloprid with β – Cyclodextrin Polymer and Analysis by HPLC


Affiliations
1 Punjabi University, Neighbourhood Campus, Rampura Phul, Bathinda (Pb.), India
2 Department of Chemistry, Punjabi University, Patiala (Pb.), India
     

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Cyclodextrins are type of oligosaccharide consisting of glucose units. Cyclodextrins are of mainly of three types α, β and γ consisting of six, seven and eight glucose units respectively joined with α-1,4-glycosodic linkage. β-cyclodextrin is a very stable, most useful and low priced oligosaccharide. Guest molecules can be incorporated in its cavity to form supramolecular complex. β-cyclodextrin is modified with cross-linker 1,4-butanedioldiglycidyl ether to form polymer. This sorbent was used as a support for the solid phase extraction of imidacloprid from water samples and these samples were analysed with the help of HPLC. For quantitatative extraction(≥95%) of imidacloprid with β - cyclodextrin polymer various parameters like pH, amount of sorbent, sample volume, contact time and volume of eluent were optimized. The HPLC analysis of imidacloprid was done at λmax at 278nm using 80:20 acetonitrile: water as a mobile phase with a flow rate 1.0ml/min. Rt for imidacloprid comes at 2.9 min. Enrichment factor is 75. LOD is 0.45 ng/ml and LOQ is 1.5 ng/ml. Relative standard deviation is 2.1%. The developed method was applied successfully for the determination of Imidacloprid in various water samples.

Keywords

β – Cyclodextrin, A-1,4-Glycosodic Linkage, Supramolecular Complex, Imidacloprid.
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  • Preconcentration of Imidacloprid with β – Cyclodextrin Polymer and Analysis by HPLC

Abstract Views: 119  |  PDF Views: 5

Authors

Vanita
Punjabi University, Neighbourhood Campus, Rampura Phul, Bathinda (Pb.), India
Usha Gupta
Department of Chemistry, Punjabi University, Patiala (Pb.), India

Abstract


Cyclodextrins are type of oligosaccharide consisting of glucose units. Cyclodextrins are of mainly of three types α, β and γ consisting of six, seven and eight glucose units respectively joined with α-1,4-glycosodic linkage. β-cyclodextrin is a very stable, most useful and low priced oligosaccharide. Guest molecules can be incorporated in its cavity to form supramolecular complex. β-cyclodextrin is modified with cross-linker 1,4-butanedioldiglycidyl ether to form polymer. This sorbent was used as a support for the solid phase extraction of imidacloprid from water samples and these samples were analysed with the help of HPLC. For quantitatative extraction(≥95%) of imidacloprid with β - cyclodextrin polymer various parameters like pH, amount of sorbent, sample volume, contact time and volume of eluent were optimized. The HPLC analysis of imidacloprid was done at λmax at 278nm using 80:20 acetonitrile: water as a mobile phase with a flow rate 1.0ml/min. Rt for imidacloprid comes at 2.9 min. Enrichment factor is 75. LOD is 0.45 ng/ml and LOQ is 1.5 ng/ml. Relative standard deviation is 2.1%. The developed method was applied successfully for the determination of Imidacloprid in various water samples.

Keywords


β – Cyclodextrin, A-1,4-Glycosodic Linkage, Supramolecular Complex, Imidacloprid.