Asian Journal of Pharmacy and Technology

Sobhy M. El-Adl ¹, Abdallah A. El-Shanawany ¹, Lobna M. Abdel-Aziz ¹, Ali F. Hassan ²

Affiliations
1 Department of Medicinal Chemistry, Zagazige University, Zagazig, EG
2 Department of Pharmaceutical Chemistry, Al-Azhar University, Assuit, EG

Abstract

New simple, accurate and sensitive spectrophotometric method for determination of Cefepimeand Captopril in bulk and dosage forms.the developed method is based on the alkaline hydrolysis of the studied drugs and subsequent reactions of the resulting hydrolysates with NBD- Cl as a chromogenic reagent to form a yellow color measured at 401 nm. This mrthod could be used for their analysis in pure forms and in pharmaceutical formulations. Effect of reagent concentration, NaOH concentration, temperature, solvent, HClconcentraion, time of hydrolysis and time after addition of reagent on the absorption was studied. Beer's law was obeyed in the range of 8-120 μg/ml for cefepime and 2-24 μg/ml for Captopril. The proposed methods were applied for determination of Cefepimeand Captopril in pharmaceutical preparations and were validated when obtained results were compared with reference methods.

Keywords

Cefepime, Captopril, NBD-Cl and NaOH.

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Mahmoud M. Sebaiy ¹, Abdullah A. El-Shanawany ¹, Sobhy M. El-Adl ¹, Lobna M. Abdel-Aziz ¹

Affiliations
1 Medicinal Chemistry Department, Zagazig University, EG

Abstract

Two simple, sensitive and extractive spectrophotometric methods have been developed for the determination of gatifloxacin, levofloxacin HCl and lomefloxacin HCl in pure form and in pharmaceutical formulations. The methods are based on the formation of yellow and blue coloured ion-pair extractable complexes between the three drugs and two dyes, bromophenol red and fast green FCF with absorption maxima at 426-430 nm and 631 nm for both dyes, respectively. The stoichiometry of the complex in either cases was found to be 1:1. Beer's law was obeyed in the range of 10-55 μg/ml for gatifloxacin and 10-70 μg/ml for levofloxacin HCl and lomefloxacin HCl in case of bromophenol red method and in the range of 0.2-1.8 μg/ml for the three drugs in case of fast green FCF method. Various analytical parameters have been evaluated and the results were validated according to ICH guidelines. The proposed methods have been applied successfully to the analysis of drugs in pure form and in its dosage forms and no interference was observed from common excipients present in pharmaceutical formulations.

Keywords

Gatifloxacin, Levofloxacin HCl, Lomefloxacin HCl, Ion-Pair Complexes, Bromophenol Red, Fast Green FCF.

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