Asian Journal of Pharmaceutical Analysis https://www.i-scholar.in/index.php/Ajpa <div id="i-scholerabout">Asian Journal of Pharmaceutical Analysis (AJPAna) is an international, peer-reviewed journal, devoted to pharmaceutical analysis.</div> en-US avpublications@gmail.com (Dr. Mrs. Monika S. Daharwal) avpublications@gmail.com (Dr. Mrs. Monika S. Daharwal) Fri, 01 Dec 2017 00:00:00 +0000 OJS 2.4.2.0 http://blogs.law.harvard.edu/tech/rss 60 Validated Simultaneous UV Spectrophotometric Methods for Estimation of Tramadol Hydrochloride and Ketorolac Tromethamine in Bulk and Marketed Capsule Formulation https://www.i-scholar.in/index.php/Ajpa/article/view/178352 The present study describes new, simple, rapid and novel spectrophotometric methods for simultaneous estimation of Tramadol Hydrochloride and Ketorolac Tromethamine in bulk and capsule formulation. The combination was analysed by simultaneous equation method (Method A), Absorbance correction method (Method B) and First order derivative spectroscopic method (Method C). Method A involved measurement of absorbance at two wavelengths, 271 nm and 246 nm, λmax of Tramadol hydrochloride and Ketorolac tromethamine respectively. For method B, wavelengths 331 nm (for determination of Ketorolac tromethamine) and 271 nm were used. For method C, wavelengths 339.2 nm (Zero crossing of Tramadol hydrochloride) and 229.4 nm (Zero crossing of Ketorolac tromethamine) were used for determination of Ketorolac tromethamine and Tramadol hydrochloride respectively. Beer’s law was obeyed in concentration range of 5-50 μg/ ml and 2-20 μg/ ml for Tramadol hydrochloride and Ketorolac tromethamine respectively by all the methods. Method C was found to be more simple and sensitive. The proposed methods are recommended for routine analysis of pharmaceutical formulations due to their specificity, rapidity, simplicity and accuracy. These methods were validated for linearity, accuracy and precision as per ICH guidelines. V. S. Tambe, M. N. Deodhar, Vijayalakshmi Prakya https://www.i-scholar.in/index.php/Ajpa/article/view/178352 Fri, 01 Dec 2017 00:00:00 +0000 Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Cefadroxil and Probencid in Bulk and Tablet https://www.i-scholar.in/index.php/Ajpa/article/view/178353 A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Cefadroxil (CEF) and Probenecid (PROB) in bulk and tablet dosage form. The separation was achieved by Princeton SPHER-100 C18 column of configuration was selected for further study. (Princeton SPHER-100 C<sub>18</sub>, 250×4.6 mm, particle size 5 μm.). CEF is freely soluble in methanol and slightly soluble in acetonitrile, water and insoluble in ethanol. Probencid is freely soluble in methanol, slightly soluble in acetonitile, water. Initially methanol and acetronitrile in different ratios were tried and then acetonitril and phosphate buffer (PH 2.5 adjusted with orthophosphoric acid). It was found that acetonitril and phosphate buffer (PH 2.5 adjusted with orthophosphoric acid). In the ratio 60:40 v/v with the flow rate of 1mL/min gives good resolution of peaks with minimum tailing as compared to other mobile phases. Detection was carried out at 242 nm. Retention time of Cefadroxil and Probenecid was found to be 2.213 and 6.62 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity for Cefadroxil was found in the range of 10- 60μg/ml and Probenecid was in the range of 10-60 μg/ml. The percentage recoveries obtained for Cefadroxil and Probenecid were found to be in range of 99.52-99.81 % and 99.50-99.53% respectively. Mayur S. Jain, Shashikant D. Barhate, Bhushan P. Gayakwad https://www.i-scholar.in/index.php/Ajpa/article/view/178353 Fri, 01 Dec 2017 00:00:00 +0000 Synthesis Of 2-(3-Substitutedamino-2, 4-Dithiobiuretoformamidino-4-Isobutoxyphenyl)-4-Methyl-5-Carboxy-1, 3-Thiazoles https://www.i-scholar.in/index.php/Ajpa/article/view/178355 A novel method has been developed for the synthesis of 2-(3-substitutedamino-2,4-dithiobiuretoformamidino-4-isobutoxyphenyl)-4-methyl-5-carboxy-1,3-thiazoles by the reaction of 2-(3-substitutedthioamidoformamidino-4-isobutoxyphenyl)-4-methyl-5-carboxy-1,3-thiazoles with substituted isothiocyanate in 60% ethanol-acetone. The method provides rapid and easy access to compounds in good yields by using 60% ethanol-acetone medium. A. S. Shendge, D. T. Tayade, P. R. Kale https://www.i-scholar.in/index.php/Ajpa/article/view/178355 Fri, 01 Dec 2017 00:00:00 +0000 Comparison Study of Conventional and Microwave Assisted Force Degradation by RP-HPLC Method of Pharmaceutical Drug and Dosage Form https://www.i-scholar.in/index.php/Ajpa/article/view/178357 Comparison Study of conventional and Microwave assisted Force Degradation by using RP- HPLC was carried for determination of Piracetam in film coated tablets. A Grace C18 (250mm x 4.6ID, Particle size: 5 micron)RP- 18 column with a mobile phase consisting of Methanol: Water(20:80v/v) was used. Microwave assisted Force Degradation was performed acid, alkali, wet heat, oxidative and dry heat degradation study to prove that similar degradation product using microwave oven reduced time as compared to conventional. Quantitative evaluation was performed at 205 nm. The HPLC method is selective, precise and accurate and can be used for routine analysis of preparations in pharmaceutical industry quality control laboratories. Sushil D. Patil, Prajkta Varpe, Sayali Chaure, Sanjay Kshirsagar https://www.i-scholar.in/index.php/Ajpa/article/view/178357 Fri, 01 Dec 2017 00:00:00 +0000 Development and Validation of Difference Spectrophotometric Method for the Quantitative Estimation of Mesalamine in Bulk Drug and Dosage Forms https://www.i-scholar.in/index.php/Ajpa/article/view/178358 A sensitive, precise, economic spectrophotometric method i.e. a difference spectroscopic method was developed for the estimation of mesalamine in bulk and dosage (tablet and suppository) forms. Difference spectroscopic method was developed by measuring the difference absorbance of the drug in 0.1N HCl and in 0.1 N NaOH showing maximum absorbance at 330nm. Beer’s law was obeyed in the concentration range of 5-25 μg/ml. The LOD and LOQ were found to be 0.3 μg/ml and 0.9 μg/ml respectively. Correlation coefficient was observed as 0.999. The method was validated as per ICH guideline Q2R1 and the results were within the official limits. The developed difference spectroscopic method was applied for quantitative analysis of the drug in bulk and pharmaceutical dosage forms. P. Ravi Kumar, Y. Padmavathi, P. Niveditha, N. Raghavendra Babu https://www.i-scholar.in/index.php/Ajpa/article/view/178358 Fri, 01 Dec 2017 00:00:00 +0000 Validated RP-Capillary HPLC-Dad Method for Simultaneous Analysis of Some Paraben Preservatives in Pharmaceutical and Cosmetics/personal Care Formulations https://www.i-scholar.in/index.php/Ajpa/article/view/178359 A fast and sensitive RP- capillary HPLC-DAD method that allows simultaneous determination of p-hydroxybenzoates commonly known as parabens in personal care formulations and pharmaceuticals is described in present study. Parabens are widely used as preservatives in cosmetics, pharmaceuticals and foods products. The separation was achieved on Agilent Zorbax SB C-18 narrow bore RR column using Acetonitrile: water as mobile phase. The results showed that separation is achieved for the target analytes within 10 min. The method was evaluated for analytical characteristics with respect to limit of detection, limit of quantification, linearity, interday and intraday precision, specificity, robustness and stability of standards. The calibration curves were linear with a correlation coefficient higher than 0.997 for each analyte. Relative standard deviation (%RSD) for inter and intraday precision studies were &lt; 3.5% for all four parabens. Methyl Paraben was used as preservative in maximum formulations. The method was successfully applied to commercially available cosmetics and personal care formulations. The extraction process reported is easy and economical. The proposed method can be successfully put on board in quality control and testing laboratories. Atul Bajaj, Cijo John, Rohitashva Mani Tripathi, Joydip Choudhury https://www.i-scholar.in/index.php/Ajpa/article/view/178359 Fri, 01 Dec 2017 00:00:00 +0000 Spectrofluorimetric Estimation of Some Fluoroquinolones https://www.i-scholar.in/index.php/Ajpa/article/view/178360 A highly sensitive spectrofluorimetric procedure is developed for the analysis of certain fluoroquinolones: norfloxacin (NFX), sparfloxacin (SFX) and ofloxacin (OFX) in their pharmaceutical dosage forms and in biological fluids. This procedure is based upon the intrinsic fluorescence of selected drugs in 0.1N H<sub>2</sub>SO<sub>4</sub>. The excitation (λext ) and emission wavelengths (λemis.) are 270 nm and 464 nm, 276 nm and 442 nm, 280 nm and 510 nm for NFX, SFX and OFX respectively. The calibration curve was rectilinear from the 0.8-2 μg mL-1, 0.4-2.8 μg mL-1 and 0.01-0.4 μg mL-1 for NFX, SFX and OFX respectively. The accuracy and precision of the proposed method was confirmed by estimating six replicates within Beer-Lambert limits for each of the investigated drug with correlation coefficient not less than 0.999. The developed method was applied successfully for the determination of the studied drugs in their pharmaceutical dosage forms and in the biological fluids with a good precision and accuracy compared to official and reported methods as revealed by statistical analysis. Rakesh Patel, Anil Bhandari https://www.i-scholar.in/index.php/Ajpa/article/view/178360 Fri, 01 Dec 2017 00:00:00 +0000 A Review on Analytical Techniques for Estimation of Anti Cholinergic Drug: Glycopyrronium Bromide https://www.i-scholar.in/index.php/Ajpa/article/view/178361 Glycopyrronium bromide is a Anticholinergic class of drug indicated for the treatment of chronic obstructive pulmonary drug. Various analytical methods used for the estimation of Glycopyrronium bromide has been reviewed in this paper. These include Ultraviolet spectrometry, High performance thin layer chromatography, High performance liquid chromatography, Ion pair, to determine the amount of Glycopyrronium bromide in bulk drugs and pharmaceutical formulations and Biological Fluids. Stability indicating and impurity profiling methods for Glycopyrronium bromide are also described. These analytical methods can be used for qualitative and quantitative estimation of Glycopyrronium bromide and / or its related impurities or degradants in bulk and formulation. Khushboo Patel, Dhara Patel, Jaya Dwivedi, Priyanka Newal, Dhananjay Meshram https://www.i-scholar.in/index.php/Ajpa/article/view/178361 Fri, 01 Dec 2017 00:00:00 +0000 Capillary Electrophoresis: A Potential Tool for Separation and Analysis-Brief Review https://www.i-scholar.in/index.php/Ajpa/article/view/178362 Capillary electrophoresis (CE) is a potential analytical and separation technique for wide variety of analytes, ranging from micro to macromolecules of biological or chemical origin belongs to either inorganic or organic class possesses simple or complex molecules. CE technology has been valuable for the comprehensive determination of drug and process related impurities. CE is a family of electrokinetic separation methods performed in capillaries and in micro-fluidic channels rely on a high voltage electric field being applied over a solution which is held in a capillary tube. CE classified according to mode of separation on the basis of differences in charge, size and frictional force, offers fast separations with excellent efficiency. CE is one of favorite analytical tool for assay of pharmaceutical API including determination of drug related impurities, as it provides reliable result in minimum time period; also, it offers a high degree of automation and requires minimal sample preparation and. CE possess other versatile applications such as chiral, stoichiometric and bioanalysis of pharmaceutical API. The current review presents basic principles underlying different modes of CE, and their application in analysis of pharmaceutical analysis. Rajesh B. Nawale, Hrishikesh H. Rajput, Mayura A. Kale, Uday A. Deokate https://www.i-scholar.in/index.php/Ajpa/article/view/178362 Fri, 01 Dec 2017 00:00:00 +0000