Asian Journal of Bio Science

S. N. Salunkhe ¹, Y. A. Deshmukh ²

Affiliations
1 College of Agriculture Biotechnology, Aurangabad (M.S.), IN
2 B.A. College of Agricultural, Anand Agricultural University, Anand (Gujarat), IN

Abstract

Genetic variability and relationship between varieties are of great importance for cotton breeding. ISSR marker systems were used for identification and genetic diversity analysis of elite G. hirsutum, G. arboreum and introgressed lines. 12 cotton genotypes were subjected to ISSR analysis using 55 ISSR primers. PCR products were subjected to agarose gel electrophoresis and the banding patterns were compared among 12 elite cotton varieties of diploid, tetraploid and introgressed cotton. Out of 55 ISSR primers tested, 15 were scorable, producing 101 marker bands with 83 being polymorphic. The primer IS-08 generated the greatest number of polymorphic markers. The ISSR markers were found to be reproducible and polymorphic. A dendrogram constructed from ISSR data classified 12 cotton genotypes into two major clusters, one containing six genotypes belonging to G. hirsutum cultivars and the other contained 4 genotypes belonging to G. arboreum cultivars. Two introgressed cultivars PAIG-8/1 and PAIG-27 showed highest level of genetic similarity with G. arboretum varieties. ISSR technique was thus found to be efficient method for detecting DNA polymorphism useful for DNA fingerprinting and genetic diversity analysis in cotton.

Keywords

Genetic Diversity, Molecular Markers, ISSR, Cotto.

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Deep Ratan Kumar ¹, Y. A. Deshmukh ², Tejbhan Saini ³

Affiliations
1 Plant Molecular Genetics Laboratory, Division of Biotechnology, M.I.T.S., Rayagada (Orissa), IN
2 Department of Agricultural Botany, Marathwada Agricultural University, Parbhani (M.S.), IN
3 Indira Gandhi Agriculture University, Raipur (C.G.), IN

Abstract

In the present investigation RAPD marker was used for the elucidation of genetic relationships in the genus Cajanus and genetic fingerprinting of pigeonpea cultivars as well as wild species of Cajanus. RAPD markers utilized for the identification of pigeonpea,   cultivars (DSLR-17, BDN-2, ICWR-03 and ICWR-12) and ten wild species, including C. cajanifolius, C. lineatus, C. sericeus, C. acutifolius, C. lanceolatus, C. reticulates, C. albicans, C. scarabaeoides, C. volubilis and C. platycarpus, using a set of 10 primers were found to be polymorphic at species level and generated 85 unequivocal scorable polymorphic bands. The size of amplification products ranges from 102 bp to 2854 bp. The present study accentuates upon the utility of RAPD markers for the identification of cultivars of pigeonpea and allied species of C. cajan. The inter/ intra specific genetic variability studies based on RAPD marker showed a large amount of genetic variation between the species of Cajanus and their clustering pattern partially, supported the sectional classification. It was hypothesised that both C. cajan and C. cajanifolius might be derived from a common ancestor and experienced minor genomic rearrangement during divergence.

Keywords

Cajanus, Pigeonpea, RAPD, Proximity Matrix Analysis.

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