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Jadhav, Vilas
- Assessment of Genetic Purity in Hybrid Lines by Field Grow-Out Test and Molecular Markers
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 106-118Abstract
Tomato is a significant vegetable crop of special economic importance, ranking second to potato in the world. As genetic composition of a variety is its ultimate identity, achieving and maintaining genetic purity in crops is an important both from agronomic as well as breeding point of view. The present investigation was carried out to identify polymorphic primers where it can distinguish the hybrid parental line and utilization of this marker for further genotyping in the hybrid population. Towards this, 18 random decamer primers (RAPD), were screened between hybrid 2 parental lines, out of which only one primer (OPAO9) showed polymorphism and also study the tomato hybrid 2 parental lines consisted of 42 plants were analysed by using SSR Marker and only one primer (TGS0100) was found to be polymorphic which showed difference in banding pattern with hybrid 2 parental lines (male and female) and to check the genetic purity through protein analysis in the given tomato hybrid 2 parental lines (male and female). It was successfully reproduced in the hybrids. Thus, the present investigation shows the efficiency of RAPD and SSR marker as a molecular tool for genetic purity analysis. Hence, this marker was employed in the hybrid population. Furthermore DUS characters were also taken in the hybrids and parental lines. The results were correlating with the molecular data. Therefore, molecular GOT can be effectively employed in hybrid lots for purity checking. This could be better and fast technology than field GOT and it will be more useful for the quality assessment in the seed industries.
Keywords
Parental Line, Genetic Purity, RAPD, SSR Marker, Biochemical Marker.
- Assessment of Genetic Purity of Tomato F1 Hybrid Seeds with Molecular and Biochemical Markers
Authors
1 Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 193-198Abstract
DNA marker is a new approach based on DNA polymorphism among tested genotypes, (John and Williams, 2008), thus, applicable to biological research. It offers many advantages over other categories of markers such as morphological, cytological or biochemical markers. For example, DNA marker can cover the whole genome and, therefore, is much larger in quantity. There is more polymorphism in DNA markers, which are able to reveal the variation and allelism. Many DNA markers are co-dominant and can differentiate between the homozygous and heterozygous genotypes. Furthermore, DNA markers are “neutral”, and they have no effect on phenotype, no epistatic effect, and are not influenced by environmental conditions and development stages. Therefore, DNA marker is simple, quick, less environmentally conditioned, and experimentally reproducible well. It has been applied widely in the identification, registration of plant variety, and in monitoring of the seed purity and the authenticity with high accuracy, high reliability and low cost. However, due to rapid developments in marker technology, statistical methodology for identifying quantitative trait loci (QTLs) and the jargon used by molecular biologists, the utility of DNA markers in plant breeding may not be clearly understood by non-molecular biologists. This review provides an introduction to DNA markers and the concept of polymorphism, linkage analysis and map construction, the principles of QTL analysis and how markers may be applied in breeding programs using MAS.