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Sharma, Vikas
- In Vitro Cytotoxic Effect of Leaves and Stem Bark of Azadirachta indica on Human Colon, Liver, Neurablastoma and Prostate Cancer Cell Lines
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Authors
Affiliations
1 Division of Biochemistry and Plant Physiology, Sher-E Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, Jammu (J&K), IN
2 School of Biotechnology, University of Jammu, Jammu (J&K), IN
1 Division of Biochemistry and Plant Physiology, Sher-E Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, Jammu (J&K), IN
2 School of Biotechnology, University of Jammu, Jammu (J&K), IN
Source
Asian Journal of Bio Science, Vol 4, No 1 (2009), Pagination: 33-37Abstract
Cancer still remains the major cause of mortality and morbidity all over the world. In the search of potential anticancer agents from the medicinal plants, the present research work was carried out to examine the anticancer properties of Azadirachta indica leaves and stem bark against human cancer cell lines via., ethanolic, hydro-ethanolic and aqueous extracts using sulphorhodamine B (SRB) dye. All the three extracts from leaf part and stem-bark showed in vitro cytotoxicity against all the human cancer cell lines at 100 μg/ml. At lower doses (10 and 30 μg/ml) aqueous extract from leaf part was found to be more active than ethanolic and hydro-ethanolic extracts in dose dependent manner. Results showed the potent anticancer effect of Azadirachta indica (leaves and stem bark) on human cancer cell lines of colon, liver, neurablastoma and prostate origin and the plant can be explored for probable anticancer lead molecules for the drug development.Keywords
Azadirachta indica, Human Cancer Cell Lines, In vitro Cytotoxicity, Anticancer.
- A Comparative Study of Quality of River Waters in the City of Allahabad, U.P., India
Abstract Views :402 |
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Authors
Affiliations
1 Division of Biochemistry and Plant Physiology, Sher-e Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, Jammu (J&K), IN
2 School of Biotechnology, University of Jammu, Jammu (J&K), IN
1 Division of Biochemistry and Plant Physiology, Sher-e Kashmir University of Agricultural Sciences and Technology of Jammu, Chatha, Jammu (J&K), IN
2 School of Biotechnology, University of Jammu, Jammu (J&K), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 296-297Abstract
River water was assayed in the city of Allahabad. Amongst the parameters used were BOD, DO, pH, conductivity and heavy metals, viz., Cu, Cd, Cr, Fe, Zn, Pb, Ni, Mn. Site specific water samples were collected from the ghats of Ganga, Yamuna, Sangam and subjected to analysis. Physico-chemical characteristics of the water samples indicated a lower level of pollution in the water of Yamuna river whereas the confluence (Sangam) was found to be most polluted.Keywords
Physico-Chemical, Heavy Metals, Water Samples, Pollution.- Isolation and Screening of Cellulase Producing Bacterial Strains from Cafeteria Waste
Abstract Views :271 |
PDF Views:6
Authors
Affiliations
1 Molecular Genetics Lab, Centre for Cellular and Molecular Biology, Amity Institute of Biotechnology,Amity University, Sector 125, Noida (U.P.), IN
2 Department of Biotechnology, National Institute of Technology, Raipur (C.G.), IN
1 Molecular Genetics Lab, Centre for Cellular and Molecular Biology, Amity Institute of Biotechnology,Amity University, Sector 125, Noida (U.P.), IN
2 Department of Biotechnology, National Institute of Technology, Raipur (C.G.), IN
Source
Asian Journal of Bio Science, Vol 13, No 1 (2018), Pagination: 28-31Abstract
Cellulases are a group of enzymes that are involved in the breakdown of cellulose into simpler sugars. These enzymes have tremendous application in the production various products like pulp and paper, textiles etc. Plants, animals and various microorganisms like protozoans, bacteria and fungi naturally produce these enzymes. Since most industrial applications utilize fungi and bacteria for fermentation, this study was also designed to isolate cellulose producing bacterial strains. In this study, food waste was collected from cafeteria and used for isolation of cellulase producing bacterial strains. The waste was pulverized using mortar and pestle subsequently used for making serial dilution, followed by the spreading of diluents on nutrient agar plates. Morphologically distinct colonies were selected and streaked to obtain single colonies. Cellulase production potential of the selected 8 isolates was tested by streaking each strain on agar plates enriched with different concentration of carboxymethylcellulose (as the sole carbon source). The plates were incubated for 72-120 hours followed by staining with 1% (v/v) Congo-red dye. Further the plates were decolourised by 1M Sodium chloride solution. A zone of clearance was observed around the bacterial strains capable of cellulase production because of their ability to hydrolyze cellulose. Further, the ability of the isolates to utilize varying concentration of CMC was tested in order to ascertain. The study resulted in isolation of 3 cellulase producing bacterial strains from cafeteria waste which can be utilized in cellulase production in the industries and also in agricultural waste management.Keywords
Cellulase, Cellulose Hydrolysis, Waste, Paper, Pulp Industry, Microbial Product.References
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