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Authors
Affiliations
1 Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar (Haryana), IN
Source
Asian Journal of Bio Science, Vol 3, No 2 (2008), Pagination: 275-278
Abstract
Immobilization of tannase from Aspergillus heteromorphus was studied on DEAE-Sephadex A-50, Chitin and Ca-alginate. Among the three methods studied, DEAE-Sephadex A-50 was observed the best support to immobilize A. heteromorphus tannase with 91.5% immobilization. On the other hand Ca-alginate and Chitin supported 79.3% and 34.5% immobilization, respectively. When the immobilized enzyme was studied for its repeated use, a constant decline in the enzyme activity was observed. However, at the end of seventh cycle, operational stability of the immobilized enzyme was 88 %, 61.7 % and 55.7 % for Chitin, Ca-alginate and DEAE-Cellulose, respectively.
Keywords
Tannase, Immobilization, Food Industries, Aspergillus heteromophus.
