Asian Journal of Bio Science

T. Devasena ¹, T. Ravimycin ²

Affiliations
1 Department of Botany, Annamalai University, Ambattur Study Centre, Chennai (T.N.), IN
2 Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal (T.N.), IN

Abstract

We investigated the antifungal property of gold Nanoparticles coated with ketoconazole against the dandruff causing organism-Malassezia pachydermatis in comparison with pure ketoconazole by disc diffusion method. The diameter of the inhibition zone is greater for gold Nanoparticles coated with ketoconazole than for pure ketoconazole. This shows that gold Nanoparticles enhances the bioactivity of ketoconazole. Zone of inhibition increased with increase in concentration of gold Nanoparticles (0.1 mM<0.2 mM<0.3 mM). We conclude that ketoconazole coated gold Nanoparticles prevent the growth of the dandruff causing organism. The inhibitory power is greater than the pure drug. Thus, gold Nanoparticles can be incorporated along with ketoconazole and formulated into an effective antidandruff shampoo.

Keywords

Nanoparticles, Gold, Dandruff, Ketoconazole, Malassezia.

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T. Devasena ¹

Affiliations
1 Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal (T.N.), IN

Abstract

1,2-dimethylhydrazine (DMH) is a toxic environmental pollutant. Humans are exposed to DMH through rocket fuel and Gyromitra species of mushroom. DMH induced colon tumor in rats mimics human colon tumor in morphological and histological aspects. Thus, DMH model is well established and correlated with human problems.The effects of dietary fenugreek seeds on induced colon tumor and oxidative stress was investigated in male Wistar rats. Rats administered with a weekly subcutaneous injection of DMH (20 mg/kg body weight) for 15 weeks developed colon tumor with 100% incidence, and showed a significant, i) decrease in lipid peroxidation (LPO) measured interms of thiobarbituric acid reactive substances (TBARS), ii) decrease in phospholipid, a major substrate for LPO and iii) increase in glutathione dependent enzymes-glutathione peroxidase (GPx) and glutathione S-transferase (GST), when compared to control rats. However, supplementation of dietary fenugreek to DMH treated rats significantly decreased the tumor incidence to 16.66%, increased the TBARS and phospholipid content and decreased the GPx and GST activities when compared to DMH treated rats. It is suggested that fenugreek act as antitumorigenic agent by influencing DMH induced colon tumor incidence and oxidative stress through its constituents flavonoids, saponin, protease inhibitors and dietary fibre.

Keywords

Fenugreek, Colon Cancer, Phospholipid, Lipid Peroxidation, Glutathione, Mushroom.

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