Asian Journal of Bio Science

B. L. Jangale ¹, N. G. Sonone ¹, T. B. Ugale ², N. R. Toke ², P. B. Jangam ³, R. S. Holkar ³

Affiliations
1 Department of Plant Biotechnology, K.K. Wagh College of Agricultural Biotechnology, Panchavati, Nashik (M.S.), IN
2 Department of Agricultural Entomology, K.K. Wagh College of Agriculture, Panchavati, Nashik (M.S.), IN
3 Department of Biotechnology, K.K. Wagh College of Arts, Science and Commerce, Panchavati, Nashik (M.S.), IN

Abstract

Medicinal plants represents rich source for antimicrobial agents and thus widely used in different countries and are a source of potent and powerful drugs. Considering the vast potentiality of medicinal plants as antibacterial agents, a systematic investigation was undertaken to screen the antibacterial activity of five medicinal plants viz. Solanum torvum, Adhatoda vasica, Terminalia chebula, Asparagus racemosus and Simarouba glauca against bacterial pathogens Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The ethanol leaf extracts of five plants showed variable zone of inhibition ranging from 3 to 11 mm against B. subtilis, S. aureus, E. coli and .P aeruginosa. The methanol extracts of all five plants failed to show zone of inhibition. However, hot water extract of S. torvum showed zone of inhibition about 6 mm against B. subtilis and P aeruginosa and about 7 mm against S. aureus and E. coli. The highest zone of inhibition was measured about 12 mm for ethanol + methanol extracts of A. vasica against E. coli. Thus, it reveals that bioactive compounds like alkaloids, flavonoids, terpenoids etc. may be the cause of inhibition and can easily be isolated in presence of ethanol, methanol, ethanol + methanol and hot water.

Keywords

Medicinal Plants, Antibacterial Activity, Zone of Inhibition, Bioactive Compounds.

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Suchita R. Ghule ¹, U. P. Barkhade ¹, M. P. Moharil ¹, T. B. Ugale ²

Affiliations
1 Department of Agricultural Entomology, Dr. Punjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN
2 Department of Agricultural Entomology, K.K. Wagh College of Agriculture, Nashik (M.S.), IN

Abstract

A laboratory investigation was conducted to find out role of detoxifying enzymes in defense mechanism of cotton cultivars against mealybugs in Insect Biotechnology Laboratory of Department of Agricultural Entomology,Dr. PDKV, Akola during 2008-09. Quantitative and qualitative studies undertaken for estimation of detoxifying enzymes like Glutathione S-transferases and esterases. GST activity was found higher in resistant variety, PKV Hy-2, followed by AKH-3614-10 (Hirsutum pigmented). The highest esterase activity was found to be present in Bunny-Bt (187.14 nM mg protein^-1 min^-1) than other cotton cultivars. PKV Hy-2 showed very lowest esterase activity (76.97 nM mg protein^-1 min^-1). Glutathione-s-transferase and esterase bands were not detected in the susceptible CAHH-231 (Pigmented hybrid) variety. Three GST isozymes were observed in AKA-8, whereas, CAHH-231 (Pigmented hybrid) did not show any isozyme. The study will be helpful in understanding the biochemical basis of mealy bug resistance in cotton. The outcome of the present investigation will act as stepping stone to develop mealy bug resistant cotton variety.

Keywords

Cotton, Detoxifying Enzymes, Esterases, GST, Host Plant Resistance, Phenococcus solenopsis.

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