Asian Journal of Bio Science

Ganapathi Bhat ¹, Sumangala Bhat ², M. S. Kuruvinashetti ²

Affiliations
1 Central Food Technological Institute, Mysore (Karnataka), IN
2 Department of Biotechnology, College of Agriculture, University of Agricultural Sciences, Dharwad (Karnataka), IN

Abstract

Thirty three isolates of Trichoderma belonging to different species which were previously screened against Sclerotium rolfsii, Fusarium oxysporum and Rhizactonia solani were used for checking chitinolytic activity using agarose plate containing glycol chitin. Differences were observed among species and isolates of the same species with respect to chitinase production after induction with colloidal chitin and chitin degradation. Among all the isolates, T. virens IABT 1010, T. koningii IABT1016, T. polysporum IABT1018 were found to be more efficient chitinase producers. In all the isolates, chitinase production started within 24 hours of induction, but maximum production reached in 48 hours. Therefore, culture filtrates taken after 48 hours of induction can be used for routine screening of the Trichoderma isolates.

Keywords

Trichoderma, Chitinolytic Activity, Glycol Chitin, Colloidal Chitin.

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P. N. Jayapadma ¹, Sumangala Bhat ¹, M. S. Kuruvinashetti ¹

Affiliations
1 Department of Biotechnology, University of Agricultural Sciences, Dharwad, Karnataka, IN

Abstract

Plant regeneration protocol through somatic embryogenesis, was developed from whole cotyledon explants of two local cultivars of chilli, Byadagi Dabbi and Sankeshwar Local. Explants cultured on Murashige and Skoog (1962) medium supplemented with different levels of thidiazuron (TDZ) (0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0mg/l) induced somatic embryos and the best response was at 3mg/l TDZ. On an average, 51.3 percent explants responded in both the genotypes with over nine embryos per explant. Complete plantlet formation occurred on MS with 3mg/ABA or 2mg/l IBA. Among the different levels of ABA, IBA and higher levels of agar tried for maturation of somatic embryos and germination, ABA 3mg/l was found to be better.

Keywords

Capscicum annuum, Chilli, Cotyledon, Somatic Embryogenesis.

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