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Lall, Rohit
- Phytoremidiation of Ceratophyllum demersum L. on Arsenate and Cadmium Exposure
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 32-36Abstract
In the present study plants of Ceratophyllum demersum L. was collected and grown for six months in large hydrophobic tubes. The effect of zinc ion concentration was studied at different concentration on plant; for 7 days in 10 per cent Hoagland media. After day 1 no significant effect was observed on plant for all concentration of zinc. After day 2, 3 and 4 change in colour from green to yellow was observed with different colour intensity. It was observed that after day 5, 6 and 7 the leaves of Ceratophyllum demersum L. become black in colour, the intensity of blacking in colour was increased as concentration of zinc ion increased.The plant showed maximum accumulation of cadmium after 7 day at 20μM concentration.The maximum level of thiol compound was observed at 10μM after 3 days. The maximum level of cysteine synthetase was observed at 10μM after 3 days. The maximum level of glutathione-S-transferase was observed at 10μM after 4 days. The maximum level of glutathione reductase was observed at 10μM after 4 days. The plant showed maximum accumulation of arsenic after 7 day at 20μM concentration. The maximum level of cysteine concentration was observed at 15μM after 4 days. The maximum reduced glutathione concentration was observed at 10μM and 20μM, respectively.
Keywords
C. demersum, Arsenat, Thiol Metabolism, Cysteine Synthase, Glutathione S-transferase, Glutathione Reductase.
- Molecular Identification of Begomovirus Causing Leaf Curl Disease in Potato Plant through PCR
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 56-60Abstract
Diseases caused by geminiviruses have long been recognized as a limitation to the cultivation of several important crops, including maize, cassava; bean, squash, potato and tomato, in tropical and subtropical regions of the world. More recently, geminivirus diseases, particularly those transmitted by whiteflies, have become an even greater threat to agriculture due to the appearance of a new and more aggressive whitefly biotype. This has renewed interest in the study of geminivirus pathogenesis and epidemiology and has stimulated work on the development of virus-resistant crop plants. Recognition of disease symptoms in field samples was done and total genomic DNA was isolated from the diseased and healthy samples. The viral genome was amplified using specific two sets of primers CP and ROJAS, was checked by agarose gel electrophoresis resulted in no amplification in case of CP and 1.2kb DNA fragments with ROJAS primer gave the confirmation of presence of DNA-A. The evidence for the presence of DNA-A was obtained from PCR amplification.