Asian Journal of Bio Science

R. Anand ¹, E. Saritha ², R. Aswini ¹, V. H. Mary Jiji ¹

Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
2 Postgraduate and Research Department of Biotechnology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN

Abstract

60 pus samples collected from Coimbatore Medical College (CMC) were processed for the isolation of Candida albicans, among which 24 (40%) were found to be positive. Anticandidal susceptibility pattern revealed that the strains were highly sensitive to fluconazole, ketoconazole; moderately sensitive to clotrimazole and but resistant to nystatin. When the same strains were evaluated for their susceptibility towards aqueous and diethyl ether herbal extracts from Allium sativum, Punica granatum, Syzium aromaticum and Cassia auriculata the results were interesting that they are highly sensitive. The maximum inhibition was found to be 49 mm with Punica granatum followed by 29mm with Allium sativum. The GC-MS analysis of both the extracts revealed the bioactive principles responsible for anticandidal activity.

Keywords

Allium sativum, Punica granatum, Syzium aromaticum, Cassia auriculata, Candida albicans.

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R. Anand ¹, E. Saritha ², V. H. Mary Jiji ¹, R. Aswini ¹

Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
2 Postgraduate and Research Department of Biotechnology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN

Abstract

Thirty wheat samples procured from storage units in different zones of Coimbatore, Tamilnadu, India were processed to isolate the predominant fungal contaminants. Heterogeneous group of fungi were enumerated by standard plate count, among which four predominant organisms namely Aspergillus flavus, Aspergillus tamorii, Rhizopus spp. and Fusarium spp. were identified by macroscopic and microscopic observations. Since reputed journal reports, continuously highlight the impact of mycotoxin production in wheat by Aspergillus flavus, the isolate was chosen and processed to examine the production of aflatoxin and further analysis and confirmation was done using Albino rats and analytical techniques such as thin layer chromatography, immunodiffusion and Immunoelectrophoresis. TLC revealed the presence of G2 type of mycotoxin at a concentration of 15 ppb.

Keywords

Aspergillus flavus, Mycotoxin, Aflatoxin, Immunodiffusion.

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R. Anand ¹, R. Aswini ¹, V. H. Mary Jiji ¹, V. Keerthana ¹

Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN

Abstract

Bacillus sps-26 isolated and characterized from dairy effluents was challenged for its ability to produce alkaline proteinase using different temperature and pH as bioparameters with a chemically defined medium containing 5% casein. The maximum enzyme production was achieved at 30.C at pH 10, which was assayed using Genov method. Although moderate enzyme level was obtained at 10.C, it can pave the way for its affordable use in detergent industries.

Keywords

Bacillus sp-26, Alkaline Proteinase, Bioparameters, Tseushida Method, Lowry Method.

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