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Anand, R.
- Screening and Characterization of Ethnopharmacological Properties of Selected Flowers and Spices Against Candida albicans
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Authors
Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
2 Postgraduate and Research Department of Biotechnology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
2 Postgraduate and Research Department of Biotechnology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 171-175Abstract
60 pus samples collected from Coimbatore Medical College (CMC) were processed for the isolation of Candida albicans, among which 24 (40%) were found to be positive. Anticandidal susceptibility pattern revealed that the strains were highly sensitive to fluconazole, ketoconazole; moderately sensitive to clotrimazole and but resistant to nystatin. When the same strains were evaluated for their susceptibility towards aqueous and diethyl ether herbal extracts from Allium sativum, Punica granatum, Syzium aromaticum and Cassia auriculata the results were interesting that they are highly sensitive. The maximum inhibition was found to be 49 mm with Punica granatum followed by 29mm with Allium sativum. The GC-MS analysis of both the extracts revealed the bioactive principles responsible for anticandidal activity.Keywords
Allium sativum, Punica granatum, Syzium aromaticum, Cassia auriculata, Candida albicans.
- Characterization of Fungal Contaminants from Wheat and the Speculation of Mycotoxin with Reference to Aflatoxin
Abstract Views :192 |
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Authors
Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
2 Postgraduate and Research Department of Biotechnology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
2 Postgraduate and Research Department of Biotechnology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 191-194Abstract
Thirty wheat samples procured from storage units in different zones of Coimbatore, Tamilnadu, India were processed to isolate the predominant fungal contaminants. Heterogeneous group of fungi were enumerated by standard plate count, among which four predominant organisms namely Aspergillus flavus, Aspergillus tamorii, Rhizopus spp. and Fusarium spp. were identified by macroscopic and microscopic observations. Since reputed journal reports, continuously highlight the impact of mycotoxin production in wheat by Aspergillus flavus, the isolate was chosen and processed to examine the production of aflatoxin and further analysis and confirmation was done using Albino rats and analytical techniques such as thin layer chromatography, immunodiffusion and Immunoelectrophoresis. TLC revealed the presence of G2 type of mycotoxin at a concentration of 15 ppb.Keywords
Aspergillus flavus, Mycotoxin, Aflatoxin, Immunodiffusion.- Effect of Growth Media pH and Incubation Temperatures on the Production of Proteinases From Bacillus sp. 26
Abstract Views :189 |
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Authors
Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 216-220Abstract
Bacillus sps-26 isolated and characterized from dairy effluents was challenged for its ability to produce alkaline proteinase using different temperature and pH as bioparameters with a chemically defined medium containing 5% casein. The maximum enzyme production was achieved at 30.C at pH 10, which was assayed using Genov method. Although moderate enzyme level was obtained at 10.C, it can pave the way for its affordable use in detergent industries.Keywords
Bacillus sp-26, Alkaline Proteinase, Bioparameters, Tseushida Method, Lowry Method.- Production of Ethanol from Ipomoea batatas using Saccharomyces cerevisiae
Abstract Views :165 |
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Authors
Affiliations
1 Department of Biotechnology, Dr. N.G.P. Arts and Science College, Coimbatore (T.N.), IN
2 Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore (T.N.), IN
3 Department of Biochemistry, Dr. N.G.P. Arts and Science College, Coimbatore (T.N.), IN
4 Department of Microbial Biotechnology, Bharathiar University, Coimbatore (T.N.), IN
1 Department of Biotechnology, Dr. N.G.P. Arts and Science College, Coimbatore (T.N.), IN
2 Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore (T.N.), IN
3 Department of Biochemistry, Dr. N.G.P. Arts and Science College, Coimbatore (T.N.), IN
4 Department of Microbial Biotechnology, Bharathiar University, Coimbatore (T.N.), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 266-269Abstract
A study was carried out on yeast fermentation of sweet potato (Ipomoea batatas) using baker’s yeast (Sacchromyces cerevisiae). The fermented sugar syrup (broth) was analyzed for yeast growth, alcohol content, reducing sugar, pH, soluble solids, volatile acids and total acidity using standard protocols. Yeast growth was also monitored. Results showed that pH values decreased with increased total acidity with concomitant increase in yeast growth (biomass) and alcohol contents of the fermenting sugar syrup. There were decreases in soluble solid contents, refractive indices of the fermenting medium. The reducing sugar in the Ipomoea batatas was lowest after 48 h of saccharification using Sacchromyces cerevisiae.The value recorded was 132 to 87 mg/100ml. Volatile acids (as acetic acids), increased with alcoholic fermentation. Fermentation of sugar syrup from Ipomoea batatas is associated with physical and chemical changes that occur in other form of fermentation alongside increased in biomass. The fermented Ipomoea batatas yielded ethanol contents of 11.5 to 53.0% (v/v).Keywords
Ipomoea batatas, Fermentation, Saccharomyces cerevisiae, Alcohol, Sugar Syrup, Biomass.- Occurrence and Distribution of Clostridium perferingens in Relation to Conventional Faecal Indicator Bacterial in Shrimp Farm
Abstract Views :147 |
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Authors
Affiliations
1 Post Graduate and Research Department of Microbiology, Dr.N.G.P. College of Arts and Science, Coimbatore (T.N.), IN
2 Post Graduate and Research Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College, Poondi, Tanjavur (T.N.), IN
3 Post Graduate and Research Department of Chemistry, Avinashilingam University, Coimbatore (T.N.), IN
1 Post Graduate and Research Department of Microbiology, Dr.N.G.P. College of Arts and Science, Coimbatore (T.N.), IN
2 Post Graduate and Research Department of Botany and Microbiology, A.V.V.M. Sri Pushpam College, Poondi, Tanjavur (T.N.), IN
3 Post Graduate and Research Department of Chemistry, Avinashilingam University, Coimbatore (T.N.), IN
Source
Asian Journal of Bio Science, Vol 3, No 2 (2008), Pagination: 361-363Abstract
Biomonitors indicate the microbiological quality of water. Though E. coli is a microbiological indicator, it cannot be considered as a perfect one. In this regard, attempts for finding a new indicator of faecal pollution in place of E. coli was carried out by performing a comparative study between E. coli and Clostridium perfringens as faecal indicator in shrimp farms. The presence of these organisms was determined by evaluating the brackish water and shrimp samples from various areas of Cochin, using MPN technique. The effect of disinfectant chlorine was examined over these organisms which revealed the sensitive nature of E. coli. Clostridium perfringens was found to be highly resistant in comparison with E. coli and thus suggesting it as a better indicator of faecal contamination in brackish water to evaluate the microbial quality of shrimp farms than E. coli.