Asian Journal of Bio Science

A. S. Patil ¹, R. H. Autade ¹, J. S. Gaikwad ¹, M. A. Chandre ¹, A. V. Kharde ²

Affiliations
1 College of Agricultural Biotechnology, Loni, Ahmednagar (M.S.), IN
2 M.G.M. College of Biotechnology, Aurangabad (M.S.), IN

Abstract

Encapsulation of in vitro derived nodal segment explants of patchouli (Pogostemon cablin Benth.) was done successfully by using 4 per cent sodium alginate gel for storage and germplasm conservation. Various concentrations of sodium alginate were tried to optimize the strength of the bead, which can give maximum conversion frequency.Various-growth regulators, and natural extracts were tested for their efficiency to regenerate healthy sprouts from encapsulated explants without vitrification. The highest frequency of shoot emergence and maximum number of shoots per bud were recorded on media supplemented with 0.5 mg/1 6- benzylaminopurine (BA). Among the natural extracts tried, 10 per cent coconut water exhibited equally good response with high frequency of shoot multiplication and broader leaves. Regenerated shoots were ischolar_mained on half strength Murashige and Skoog (MS) medium devoid of growth regulators. Plants retrieved from the encapsulated buds were hardened and established in soil. The effect of storage period and temperature on the conversion frequency of encapsulated buds was studied. This technology can be adopted for ex-situ germplasm conservation of high yielding varieties of patchouli.

Keywords

Essential Oil, Growth Regulators, Natural Extracts, Multiple Shoots, Somaclonal Variations, Propagation.

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R. H. Autade ¹, A. S. Patil ¹, D. K. Jadhav ¹, Y. Sutar ¹, A. M. Bhosale ¹

Affiliations
1 Department of Plant Biotech, College of Agricultural Biotechnology, Loni, Ahmednagar (M.S.), IN

Abstract

Biofertilizer has been acknowledged as a substitute to chemical fertilizer to increase soil fertility and crop production in sustainable farming. Most of the farmers assume that chemical fertilizer gives more yield than the biofertilizer, ignoring environmental and long term losses. This study is done to test the efficiency and efficacy of the biofertilizer in opposition to the chemical fertilizer and in additions to this comparison also done among the four species of Azotobacter for their aid in increase in yield and biomass of wheat crop. The field experiment was conducted during Rabi 2006-07 season using randomized Block Design and Trimbak variety were used for sowing in 21 plots. About nine parameters of wheat crop were selected and intermittently readings were taken at 30, 60, 90 days of interval to observe alteration, due to use of biofertilizer, chemical fertilizer and some plots were kept as a control for comparison. ANOVA were used to test the significance in treatments.

Keywords

Biofertilizer, Azotobacter, Wheat, Chlorophyll.

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