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Singh, Poonam
- Phytoremidiation of Ceratophyllum demersum L. on Arsenate and Cadmium Exposure
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 32-36Abstract
In the present study plants of Ceratophyllum demersum L. was collected and grown for six months in large hydrophobic tubes. The effect of zinc ion concentration was studied at different concentration on plant; for 7 days in 10 per cent Hoagland media. After day 1 no significant effect was observed on plant for all concentration of zinc. After day 2, 3 and 4 change in colour from green to yellow was observed with different colour intensity. It was observed that after day 5, 6 and 7 the leaves of Ceratophyllum demersum L. become black in colour, the intensity of blacking in colour was increased as concentration of zinc ion increased.The plant showed maximum accumulation of cadmium after 7 day at 20μM concentration.The maximum level of thiol compound was observed at 10μM after 3 days. The maximum level of cysteine synthetase was observed at 10μM after 3 days. The maximum level of glutathione-S-transferase was observed at 10μM after 4 days. The maximum level of glutathione reductase was observed at 10μM after 4 days. The plant showed maximum accumulation of arsenic after 7 day at 20μM concentration. The maximum level of cysteine concentration was observed at 15μM after 4 days. The maximum reduced glutathione concentration was observed at 10μM and 20μM, respectively.
Keywords
C. demersum, Arsenat, Thiol Metabolism, Cysteine Synthase, Glutathione S-transferase, Glutathione Reductase.
- Molecular Identification of Begomovirus Causing Leaf Curl Disease in Potato Plant through PCR
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 56-60Abstract
Diseases caused by geminiviruses have long been recognized as a limitation to the cultivation of several important crops, including maize, cassava; bean, squash, potato and tomato, in tropical and subtropical regions of the world. More recently, geminivirus diseases, particularly those transmitted by whiteflies, have become an even greater threat to agriculture due to the appearance of a new and more aggressive whitefly biotype. This has renewed interest in the study of geminivirus pathogenesis and epidemiology and has stimulated work on the development of virus-resistant crop plants. Recognition of disease symptoms in field samples was done and total genomic DNA was isolated from the diseased and healthy samples. The viral genome was amplified using specific two sets of primers CP and ROJAS, was checked by agarose gel electrophoresis resulted in no amplification in case of CP and 1.2kb DNA fragments with ROJAS primer gave the confirmation of presence of DNA-A. The evidence for the presence of DNA-A was obtained from PCR amplification.
Keywords
Geminiviruses, PCR, Leaf Curl, Begomovirus.- Combined Mutagenic Improvement of Bacillus licheniformis SK7 for Cost-Effective Protease Production
Authors
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Microbial Biotechnology Laboratory, University Institute of Engineering and Technology, M.D. University, Rohtak (Haryana), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 91-94Abstract
The strain improvement of developed Bacillus licheniformis SK7 was achieved with the combination of physical and chemical agents i.e., UV + NTG + EMS by gradually mutation and positive selection. In case of UV light 99 per cent killing for successful selection of mutants was 6 min and 10 cm distance from source, while in case of EMS and NTG the concentration found effective was 200 μM, 30 μM, respective for 15 min. The mutant Bacillus licheniformis SK7 SN 43 was successfully developed and found stable having higher production of protease (662 U/ml) under optimized medium and physical conditions of fermentation than the wild type Bacillus licheniformis SK7 (472 U/ml) under the same conditions of fermentation.
Keywords
Mutagenesis, NTG, UV Light, Strain Improvement, Protease.- Detection of Polymorphism of ATM Gene in Leukemia
Authors
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 100-105Abstract
The Ataxia telangiectasia mutated gene encodes the ATM protein, a key element in the DNA damage response (DDR) signalling pathway responsible for maintaining genomic integrity within the cell. In order to study the genetic changes of ATM gene in Lukaemia, we collected the blood samples of healthy persons for control and leukaemia persons for disease samples. We isolated DNA and performed PCR to analyse by Gene sequencing. As a result we found less number of sequencing result in nucleotide BLAST due to which we are unable to detect the compared result of mutation. Larger and/or combined association studies are needed to conclude the result.
Keywords
ATM Gene, Gel Eelectrophoresis, Polymerase Chain Reaction, DNA Sequencing.- Isolation and Optimization of Lipase Producing Bacteria from Oil Contamination Soils
Authors
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 131-135Abstract
Lipolytic bacteria were isolated from oil contaminated soil and grown on glycerol tri-butyrate media. The lipase activity is shown by reacting with various chemicals. Lipases are glycerol ester hydrolases that catalyze the hydrolysis of triglycerides to free fatty acids and glycerol. Bacterial lipase producers were isolated from oil spilled soil from vegetable oil processing factories. The effect of incubation time, medium pH, temperature, agitation, inoculums concentration, carbon source and nitrogen source for the lipase production was studied. The lipase production was maximum at pH 7, temperature by the lipase producing bacteria Staphylococcus. Increased enzymatic production was obtained when the organisms were cultured in medium supplemented with 1per cent protease peptone by Staphylococcus. The results of the present study was to demonstrate that the micro-organism is ideal for extracellular lipase production at industrial level. Different media parameters were optimized for maximal enzyme production. Lipases activity shown on starch containing agar media at pH-7.0 and temperature at 370C for 24-48 h.