Asian Journal of Bio Science

N. Gupta ¹, J. Sabat ¹

Affiliations
1 Regional Plant Resource Centre, Bhubaneswar (Orissa), IN

Abstract

An attempt Was made to evaluate the salt tolerance behaviour of Aspergillus sp. and Bacillus sp. isolated on Brain heart infusion agar from muddy water of Bhitarkanika mangrove ecosystem by growing them under different salt concentration in broth medium . It was observed that fungus could tolerate salt stress upto 16% where as bacteria could be able to grow upto 11 %. The Aspergillus sp. showed increase in growth with the increasing concentration of NaCl where as bacteria declined its growth in this condition. It was observed that acidic metabolites was produced by fungus, therefore, its pH was decreased with the increasing level of NaCl . Simultaneously, bacteria did not show any significant changes in pH of filtrate of different saline treated cultures. The protein content in the culture filtrate of fungus increased in higher concentration of salt . However, bacteria did not behave in the same manner rather showed negative effect of salinity on growth. The present attempt made to analyze the salt stress in fungus and bacteria was of primary nature that provided us various clues to work further.

Keywords

Mangrove, Saline, Fungi, Bacteria, Salt Tolerant, NaCl, Halotolerant.

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J. Sabat ¹, N. Gupta ¹

Affiliations
1 Microbilogy laboratory, Division of Biotechnology, Regional Plant Resource Centre, Bhubaneswar (Orissa), IN

Abstract

Sixty isolates of Streptomyces were isolated from heavy metal contaminated soils of different mines of Orissa.. These isolates were studied for their gram staining properties, extra cellular and fermentative activity. All cultures were branching and rods, gram positive. They showed varied performance during the biochemical and enzymatic tests and analysis. The importance of these isolates is discussed with respect to their distribution in such a extreme habitat of heavy metal contaminated soils of mines of Orissa.

Keywords

Streptomyces, Mines, Matal Contaminated Soil.

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J. Sabat ¹, N. Gupta ¹

Affiliations
1 Microbiology Laboratory, Division of biotechnology Regional Plant Resource Centre, Bhubaneswar 751 015 (Orissa), IN

Abstract

Seven fungi were isolated from heavy metal mine soils of Joda (Orissa) , India. These fungi exhibited tolerance to manganese salt in broth culture under laboratory conditions. The growth of Aspergillus sp. 3 and Cunnighamella sp. was found to be more in 0.2% concentration of manganese.

Keywords

Manganese, Metal Tolerance, Fungi, Bacteria , Growth.

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