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Thakur, Mansee
- Evaluation of Homeopathic Drugs on Glucocorticoid Induced Osteoporosis (GIOP) Zebrafish Model
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PDF Views:9
Authors
Affiliations
1 Department of Medical Genetics, MGM School of Biomedical Sciences, MGM Medical College Building, MGM Institute of Health Sciences, Kamothe, Navi Mumbai (M.S.), IN
2 Department of Medical Biotechnology and Central Research Laboratory, MGMCET, MGM School of Biomedical Sciences, MGM Medical College Building, MGM Institute of Health Sciences, Kamothe, Navi Mumbai (M.S.), IN
1 Department of Medical Genetics, MGM School of Biomedical Sciences, MGM Medical College Building, MGM Institute of Health Sciences, Kamothe, Navi Mumbai (M.S.), IN
2 Department of Medical Biotechnology and Central Research Laboratory, MGMCET, MGM School of Biomedical Sciences, MGM Medical College Building, MGM Institute of Health Sciences, Kamothe, Navi Mumbai (M.S.), IN
Source
Asian Journal of Bio Science, Vol 13, No 1 (2018), Pagination: 32-38Abstract
Background: Homeopathic remedies are proposed as source for traditional preventive medicines. In this context our study focuses to evaluate efficacy of homoeopathic drug with reference to GIOP in Zebrafish vertebrate model. The overall approach behind this study was to determine whether homeopathic drugs can be used along with of allopathic medicines as an alternate therapy. Methods: In the present study, an attempt is made to find out the efficacy of homoeopathic medicines-Argentum Metallicum, Calcaria Carbonica and Sepia in two dilutions 6CH and 30 CH on GIOP model of zebra fish in two dilutions. Exposure was studied at 3 different points - 4, 14 and 28 days exposure (treatment with test drugs) and staining intensities were measured using Image J software. Control groups used were untreated control larvae, GIOP model untreated post 11 dpf and GIOP model treated with Alendronate. The medium was changed every day at the same time. Staining intensities measured for Alizarin Red and Calcein dye stained images. Results: Staining intensities of Alizarin red and Calcein staining of the treated group on statistical analysis showed that the means of GIOP and other treated groups were not statistically significant at p<0.05 except in the case of 28 day study for Calcarea Carbonica 30C and Sepia 30C. Though screening was processed using Alizarin red and Calcein staining, our quantification screening with calcein labeling indeed facilitated the process and diminished labor of handling. Conclusion: Our findings show that, despite some physiological differences between mammals and teleosts, the zebra fish represents an effective model for screening of bone defects. This model has been used for preliminary studies on homeopathy drugs prescribed for treatment of Osteoporosis. The proposed calcein staining protocol can represents a powerful tool for in vivo monitoring of mineralized structures.Keywords
Homeopathy, Osteoporosis, Zebra Fish, Prednisolone, Alizarin Red, Calcein.
References
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- Green Synthesis and Characterisation of Iron Oxide Nanoparticles Using Hydroponically Grown Spinach Plant Extract
Abstract Views :290 |
PDF Views:8
Authors
Affiliations
1 Department of Medical Biotechnology, MGM School of Biomedical Sciences, MGMIHS, Kamothe, Navi Mumbai, (M.S.), IN
2 Department of Medical Biotechnology and Central Research Laboratory, MGMCET, MGM School of Biomedical Sciences, MGM Medical College Building, MGM Institute of Health Sciences, Kamothe, Navi Mumbai (M.S.), IN
1 Department of Medical Biotechnology, MGM School of Biomedical Sciences, MGMIHS, Kamothe, Navi Mumbai, (M.S.), IN
2 Department of Medical Biotechnology and Central Research Laboratory, MGMCET, MGM School of Biomedical Sciences, MGM Medical College Building, MGM Institute of Health Sciences, Kamothe, Navi Mumbai (M.S.), IN
Source
Asian Journal of Bio Science, Vol 13, No 1 (2018), Pagination: 44-49Abstract
Recent advances in Nanoscience and Nanotechnology radically changed the way we diagnose, treat, and prevent various diseases in all aspects of human life. Iron oxide nanoparticles (IONPs) are one of the most vital and fascinating nanomaterial among several metallic nanoparticles that are involved in biomedical applications. IONPs have been focused on budding applications in magnetic resonance imaging, drug delivery across biological barriers or in cancer treatment by magnetic field-induced hyperthermia. In this article, we discuss the green synthesis and characterization of IONPs using hydroponically grown spinach plants leaf extract. The use of plants in the green synthesis of nanoparticles emerges as a cost-effective and eco-friendly approach. Characterization of nanoparticles was done using different methods, which include; Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), Energy Dispersive Spectroscopy analysis (EDS) and Atomic Absorption Spectroscopy (AAS). Fourier transform infrared spectroscopy identifies the functional groups of active components presents on the surface of nanoparticles. The crystalline nature of the particles was validated from an X-ray diffractometer. The size and stability were detected using SEM-EDS analysis. Iron content was found to be 40.34% by AAS.Keywords
Green Synthesis, IONPs, Spinach Leaf Extract, Hydroponic Method.References
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