Asian Journal of Bio Science

Madhulika Bhagat ¹, Jatinder Singh Arora ², Ajit Kumar Saxena ³

Affiliations
1 School of Biotechnology, University of Jammu, Jammu (J & K), IN
2 Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar (Punjab), IN
3 Pharmacology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi (J & K), IN

Abstract

Calotropis procera (Ait.) R.Br., a popular medicinal species in Indian system of medicine, is widely used for the treatment of various diseases. In the present investigation the cytotoxicity of the three extracts and their fractions from aerial parts of Calotropis procera was evaluated against central nervous system (SNB-78) human cancer cell line. The results showed that hydro-alcohloic extract has more potential at three different concentrations of 10, 30 and 100 μg/ml, among all the three extracts viz., alcoholic, hydro-alcoholic and aqueous extracts. On evaluation of the various fractions, n-butanol fraction of both hydro-alcoholic and alcoholic extracts had shown antiproliferative activity against human cancer cell line of central nervous system (SNB-78) at three different concentrations of 10 and 30 μg/ml. Aqueous extract was found to be least active.

Keywords

Calotropis Procera, Aerial Parts, Extracts, Fractions, Cytotoxicity, Human Cancer Cell Line.

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Madhulika Bhagat ¹, Jatinder Singh Arora ², Ajit Kumar Saxena ³

Affiliations
1 Department of Biotechnology, University of Jammu, Jammu (J&K), IN
2 Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar (Punjab), IN
3 Pharmacology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu (J&K), IN

Abstract

Ficus hispida Linn. is explored for its in vitro cytotoxicty against oral (KB) and colon (COLO 205) human cancer cell lines. Three extracts (alcoholic, hydro-alcoholic and aqueous) and four fractions (n-hexane, chloroform, n-butanol and aqueous) from the leaves were prepared and were evaluated using sulforhodamine B assay at 10, 30 and 100 μg/ml. The growth inhibition demonstrated by all extracts and fractions were in dose dependent manner. The alcoholic extract was most active followed by aqueous and 50% aqueous-alcoholic extract. Further on the evaluation of fractions, n-butanol fraction was highly significant among the four fractions of alcoholic extract against both oral and colon cancer cell lines.

Keywords

Ficus hispida, Cytotoxicity, SRB Assay, KB Human Cancer Cell Lines.

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