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Vakharia, D. N.
- Accessing Genetic Variability in Chickpea (Cicer arietinum L.) Varieties Differing in Susceptibility To Fusarium oxysporum F.sp. ciceri Using Issr Markers
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Authors
Affiliations
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, IN
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, IN
Source
Asian Journal of Bio Science, Vol 8, No 2 (2013), Pagination: 165-170Abstract
Due to Fusarium wilt the production of chickpea has been affected in large. Molecular variability encompassed in chickpea offers an efficient means of exploiting disease resistance variety. The DNA-based molecular marker technique, inter simple sequence repeat (ISSR) was used to study the genetic variability for 5 chickpea varieties differing in their susceptibility to wilt disease using a different ISSR primers in present investigation. From the sixteen ISSR primer used, only two primers, primer 6956-022 (sequence of (GA)8 CTC) and 6956-030 ((AC)8 YG) gave three specific amplicon viz.,167,191, 272 and 196, 249, 408 base pair (bp) respectively, for resistant variety WR-315. Similarly, 6956-029 (146 bp), 6956-022 (311 bp), 6956-038 (1157 bp), 6956-031 (119, 139 bp) and 6956-033 (310 bp) markers associated with the chickpea variety JCP-27. For susceptible variety JG-62, three ISSR primer of a sequence (GA)8 T, (AG)8YT and (AC)8CCTA generated unique band for DNA produced 1343, 534 and 231 bp markers, respectively.Keywords
Chickpea, Fusarium Oxysporum F.sp. Ciceri, Issr
References
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- Induced Resistance and Phenolic Acid Accumulation in Biological Control of Chickpea Wilt by Pseudomonas fluorescens
Abstract Views :387 |
PDF Views:0
Authors
Affiliations
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, IN
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, IN
Source
Asian Journal of Bio Science, Vol 8, No 2 (2013), Pagination: 184-188Abstract
HPLC analysis of chickpea (Cicer arietinum) plant at various wilt disease developmental stages challenged with Fusarium oxysporum f.sp ciceri indicates the plants contain high amount of phenolics when the seed was treated with Pseudomonas fluorescens local isolates Pf-3. This biocontrol bacterial isolate induced the synthesis of specific phenolic acids like salicylic acid, chlorogenic acid etc. with varied amounts at different disease development stages of wilt in chickpea. Vannilic, chlorogenic and hydroquinone were also found higher in plants treated with Fusarium oxysporum f.sp. ciceri.All the above mentioned phenolics was found higher amount in resistant variety than susceptible variety. The induction of antifungal phenolic acids in chickpea plant in the present investigation due to the application of the local isolate Pf-3 provides a biochemical basis of induced resistance in chickpea against wilt disease.Keywords
Chickpea, Fusarium oxysporum F.sp. Ciceri, HPLC, Phenolics, Pseudomonas fluorescens, Salicylic AcidReferences
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