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Ali, Arif
- Plasmid-Borne Mercury Resistance in Aquatic Escherichia coli
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Authors
Affiliations
1 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN
2 Divison of Post Harvest Technology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar (J&K), IN
3 S.M.V.D. University, Katra, Jammu (J&K), IN
1 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN
2 Divison of Post Harvest Technology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar (J&K), IN
3 S.M.V.D. University, Katra, Jammu (J&K), IN
Source
Asian Journal of Bio Science, Vol 4, No 2 (2010), Pagination: 149-152Abstract
Bacteria (E. coli) isolated from different aquatic bodies of India were analyzed for their tolerance to mercury (HgCl2 ). Out of the 30 isolates of E. coli, collected from water samples of four geographically distinct regions and hospital settings in India, 8 strains showed significantly high levels of tolerance to the inorganic form of mercury i.e mercury chloride (HgCl2). All the eight strains revealed the presence of a plasmid of approximately 24kb, and transformation of the isolated plasmids into the mercury-sensitive competent cells of E. coli DH5 rendered the transformants resistant to the same concentration of mercury as wild type-strains.Keywords
Escherichia Coli, Mercury Resistance, Mercuric Reductase (merA) Gene.- Cloning of Mercuric Reductase (merA) Gene Isolated from Wild Strains of Escherichia coli
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Authors
Affiliations
1 Jammu and Kashmir Entrepreneurship Development Institute, JLN Udhoyg Bhawan, Railhead Complex, Jammu (J&K), IN
2 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN
1 Jammu and Kashmir Entrepreneurship Development Institute, JLN Udhoyg Bhawan, Railhead Complex, Jammu (J&K), IN
2 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN
Source
Asian Journal of Bio Science, Vol 5, No 1 (2010), Pagination: 80-83Abstract
Bacterial plasmids encode resistance systems for toxic metal ions including Hg2+ functioning by energy – dependent efflux of toxic ions. The inducible mercury resistance (mer) operon encodes both a mercuric ion uptake and detoxification enzymes. In Gram-negative bacteria especially in E. coli, a periplasmic protein, MerP, an inner-membrane transport protein, MerT, and a cytoplasmic enzyme, mercuric reductase (the merA protein), are responsible for the transport of mercuric ions into cell and their reduction to elemental mercury, Hg°. Phytoremediation involves the use of plants to extract, detoxify and/or sequester environmental pollutants from soil and water. Transgenic plants cleave mercury ions from methyl-mercury complexes; reduce mercury ions to the metallic form; take up metallic mercury through their ischolar_mains; and evolve less toxic elemental mercury. PCR were performed to detect 1695 bp of mercuric reductase gene (merA), which is mainly responsible for the conversion of mercuric (Hg+2) and mercurous (Hg+1) ions into non-toxic elemental mercury. PCR products of putative merA genes form environmental E.coli strains were purified and cloned into a suitable plant expression vector like pB1121 or pCAMBIA. The construct will be transformed in calli of Nicotiana plants. Expression of merA gene in transgenic plants might provide an ecologically compatible approach for the remediation of mercury pollution.Keywords
Mercury Resistance, E. coli, PCR Amplification, Nicotiana.- Plasmid-Borne Mercury Resistance in Aquatic Escherichia coli
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Authors
Tanveer A. Shah
1,
Arif Ali
1
Affiliations
1 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN
1 Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, IN