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Charan, Amit Alexander
- Comparative Analysis of Antibacterial and Antioxidant Activity of Coriandrum sativum, Mentha pipereta and Spinacia oleracea
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Authors
Affiliations
1 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., IN
1 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., IN
Source
Asian Journal of Bio Science, Vol 8, No 2 (2013), Pagination: 205-208Abstract
The present study was carried out to evaluate the antibacterial and antioxidant activity of the crude ethanolic an acetone extracts of Mentha pipereta, Spinacia oleracea and Coriandrum sativum. The agar well diffusion assay and MIC test were carried out against four strains of bacterial species, viz., Staphylococcus aureus, E. coli, Bacillus pumulis, and Klebsiella pneumonia to determine the sample's antibacterial activity. The extracts of the plants at a concentration of 50 ?l/disc (200 mg/ml) showed minimum to moderate activity against bacteria indicating a broad spectrum activity. Variable concentrations of ethanolic extract of plant samples were effective against various pathogenic bacteria in MIC test. The result indicated the potential usefulness of these plants especially, in treating bacterial infections and justified the need for further investigations and characterization of the bioactive compounds present in the ethanolic and acetone extracts of the plants. On comparing the three plant materials, the chlorophyll content (both Chl A and Chl B) was found to be maximum in Spinacia oleracea leaves. The carotene content was found to be maximum in acetone extract of Mentha pipereta leaves, while minimum in Coriandrum sativum. Recently, attention has focused on phytochemicals as new sources of natural antioxidants. Therefore, the ethanolic crude extracts of the plant samples were screened for total phenols, flavonoids, and free radical scavenging activity. Free radical scavenging activity was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Significant differences in DPPH scavenging activity were found between the species investigated, ranging from 12.71 per cent to 68 per cent. The highest radical scavenging activity was observed in Coriandrum sativum (68% inhibition), followed by Mentha pipereta (61.62%) and Spinacia oleracea (54.72%). The total phenol content of the investigated species ranged from 26 to 75 mg CE/g extract, while flavonoids content ranged from 22 to 24 mg CE/g extract. The findings indicated promising antioxidant activity of crude extracts of the above plants and needs further exploration for their effective use in both modern and traditional system of medicines.Keywords
Mentha Pipereta, Spinacia Oleracea, Coriandrum Sativum, Staphylococcus Aureus, E. Coli, Bacillus Pumulis, Klebsiella Pneumonie, Flavonoids
References
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- Zheng,W. and Wang, S.Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. J. Agric. & Food Chem., 49(11): 5165-5170.
- Comparative Analysis of Primary Metabolites of Medicinal Plants
Abstract Views :264 |
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Authors
Affiliations
1 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture Technology and Sciences, Allahabad, U.P., IN
2 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture Technology and Sciences, Allahabad, U.P.
1 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture Technology and Sciences, Allahabad, U.P., IN
2 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture Technology and Sciences, Allahabad, U.P.
Source
Asian Journal of Bio Science, Vol 8, No 2 (2013), Pagination: 289-290Abstract
Plants have been used for the treatment and cure of many diseases and physical ailments because of their therapeutic properties. These plants are designated as medicinal plants. The goal of the present work was to estimate primary metabolites such as protein, total phenols, lipids, chlorophyll and soluble sugar in the leaves of different plant species such as Azadirachata indica, Ocimum sanctum and Mentha arvensis. Higher amount of soluble sugar was observed in the Mentha arvensis i.e. 24 per cent. Highest content of chlorophyll was observed in leaves of Azadirachata indica i.e. 1.64 per cent. Maximum amount of Protein was estimated in Mentha arvensis i.e. 27.80 per cent. Highest amount of Lipids was calculated in Azadirachata indica i.e. 0.024 per cent. Maximum amount of Phenols was found in Mentha arvensis i.e. 1.013 per cent.Keywords
Protein, Total Phenols, Lipids, Chlorophyll, Soluble SugarReferences
- Bligh, E.G. and Dyer, W.J. (1959). Biochemical methods of plant physiology. J. Biochem. & Physiol., 37: 911.
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- Profiling of Antioxidant Enzymes in Cat Fish (Clarias batrachus) Exposed to Phenolic Compounds
Abstract Views :260 |
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Authors
Affiliations
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), IN
2 Department of Biochemistry and Biochemical Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), IN
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), IN
2 Department of Biochemistry and Biochemical Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, ALLAHABAD (U.P.), IN
Source
Asian Journal of Bio Science, Vol 10, No 1 (2015), Pagination: 6-14Abstract
Pollution of water sources due to chemicals plays a primary role in the destruction of ecosystems but chemical analyses alone may not suffice to describe the adverse effects of the complex mixtures of chemicals present at contaminated sites. The potential utility of biomarkers for monitoring both environmental quality and the health of organisms inhabiting in the polluted ecosystems has received increasing attention during the last years. In the present investigation, the antioxidant profile of Clarias batrachus, a fresh water fish was determined by evaluation of antioxidant enzymes, SOD activity, catalase activity, glutathione peroxidase activity, glutathione-S-transferase activity, reduced glutathione, level of conjugated dienes, hydrogen peroxidase activity and malondialdehyde level. The studies showed that in fishes after exposure to phenolic compounds (treated group), there was a significant abnormal level of all the parameters as mentioned above at P<0.05 in comparison to normal (untreated group).Keywords
Clarias batrachus, Phenolic Compounds, Antioxidant Profile, Antioxidant Enzymes.- Citric Acid Production from Pre-Treated Sugarcane Bagasse by Aspergillus niger under Solid State Fermentation
Abstract Views :370 |
PDF Views:2
Authors
Affiliations
1 Department of Biochemistry and Biochemical Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
1 Department of Biochemistry and Biochemical Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 10, No 2 (2015), Pagination: 162-166Abstract
Citric acid is one of the important commercially produced organic acid. The aim of the present work was to study the utilization of pre-treated sugar cane bagasse for citric acid production using Aspergillus niger under solid state fermentation. The maximum value of citric acid was observed in acid treated substrate followed by urea and heat respectively. Fermentation conditions were also optimized and maximum production of citric acid occurred when the pH, Initial moisture content and temperature of the fermentation medium were 5, 65 per cent and 30°C, respectively. As a globally required organic acid for various industrial applications, citric acid can be produced at large scale by utilizing pre-treated agro residues such as sugarcane bagasse. Application of agro residues in the production of value added product can be a positive step towards agricultural waste management.Keywords
Citric Acid, Aspergillus niger, Pre-Treatment, Solid State Fermentation.References
- Ali, S., Haq, I.U., Qadeer, M.A. and Iqbal, J. (2002). Production of Citric acid byAspergillus niger using cane molasses in a stirred fermenter. Electronic J. Biotechnol., 5 (3) : 125-130.
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- Identification of Vibriospecies from Various Water Samples of Bareilly, Using Multiplex PCR
Abstract Views :241 |
PDF Views:2
Authors
Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 2 (2016), Pagination: 281-283Abstract
The purpose of this study was to investigate the prevalence of Human pathogenic vibrio species in water bodies of Bareilly, U.P. using the traditional culture and multiplex PCR methods. Vibrio species are very much prevalent in water bodies of tropical region. The study was conducted on 25 various water samples collected from rivers and ponds of Bareilly, U.P. For this, species specific primers were designed targeting the toxgene of the five pathogenic species. Confirmation was done using multiplex PCR technique for rapid detection of the five selected pathogenic species including V. cholera, V. alginolyticus, V. parahaemolyticus, V. mimicus and V. vulnificus. Out of the five targeted species three were present i.e. V. cholera, V. alginolyticus, V. parahaemolyticus. Water is essential to life, but many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections. In this review a general characterization of the most important bacterial diseases transmitted through water like cholera is present.Keywords
TCBS, Water Samples, Vibrio cholerae.References
- Chakraborty, S., Mukhopadhyay, A.K., Bhadra, R.K., Ghosh, A.N., Mitra, R. and Shimada, T. (2000). Virulence genes in environmental strains of Vibrio cholerae. Appl. Environ. Microbiol., 66 : 4022-4028.
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- To Study the Therapeutic Role of Indian Spices in the Treatment of Gastrointestinal Disease Caused by Vibrio Species
Abstract Views :225 |
PDF Views:2
Authors
Affiliations
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 12-16Abstract
Vibrio causes cholera with other major gastrointestinal disease which is very fatal and in this study we have analyzed whether the medicinal effects of these spices, which are used on daily basis, can minimize the activity of Vibrio species. In project named, “To study the therapeutic role of Indian spices in the treatment of gastrointestinal disease caused by Vibrio species” five of the spices were selected. They were Hing (Ferula assa), Jeera (Cuminum cyminum), black pepper (Piper nigrum), Saunf (Foeniculum vulgare), black mustard (Brassica nigra) based on the studies and research made on their medicinal values. In order to determine the antimicrobial activity/effect of spices bacteria Vibrio and its species were selected. The antibacterial activity of the extracts of all the spices were screened by analyzing the effect on the growth of Vibrio species through their zone of inhibition produced. Aqueous effects of all five spices were obtained using two solvents ethanol and methanol extraction with the concentration of 85 per cent and 100 per cent. The liquid portion of extract was collected and rest was discarded. Antibacterial studies were investigated using agar well diffusion method to determine the effect of these spices against the Vibrio species. The study confirmed the antioxidant activity and property of spices extracted.
Keywords
Vibrio, Ferula assa, Cuminum cyminum, Piper nigrum, Foeniculum vulgare, Brassica nigra, Syzygium aromaticum.- Optimization Studies for Algae Biofuels Production
Abstract Views :185 |
PDF Views:2
Authors
Prateek Singh
1,
Dheeban Chakravarthi Kannan
2,
Vineeta Gurnani
1,
Aradhana Irene Charan
1,
Amit Alexander Charan
1
Affiliations
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 The Energy and Resources Institute, New Delhi, IN
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 The Energy and Resources Institute, New Delhi, IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 42-48Abstract
Continuous use of fossil fuel is globally considered as unsustainable because of depleting supplies and these fuels also account for accumulation of green house gases in the environment. Renewable, carbon neutral, transport fuels are necessary for environmental and economic sustainability. Microalgae feedstocks are gaining interest in the present day energy scenario due to their fast growth potential coupled with relatively high lipid, carbohydrate and nutrients contents. All of these properties render them an excellent source for biofuels such as biodiesel, bioethanol and biomethane; as well as a number of other valuable pharmaceutical and nutraceutical products. It’s a carbon neutral fuel. But high production cost is still a big hurdle in its commercialisation. Various optimisations are discussed in this research for the commercialization of algal biofuels. The production cost of algal biofuel is still quite high, so a lot of optimisation studies for growth parameters, lipid productivity and lipid extraction process are needed, which are discussed in this study. Commercialization of microalgae for biodiesel is technically feasible. Studies had shown that algae biofuel has the potential to completely displace liquid fuels derived from petroleum. Economics of producing microalgal biodiesel need to improve substantially to make it competitive with petrodiesel, but the level of improvement necessary appears to be attainable.
Keywords
Microalgae, Biofuels, Nutraceutical, Biomass, D. tertiolecta, D. salina.- Binding Pattern Determination for Class of Anti-Alzheimer's Compound
Abstract Views :211 |
PDF Views:2
Authors
Affiliations
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 66-70Abstract
Alzheimer’s disease (AD) is the most common form of dementia. No cure has been observed for the disease, and it worsens as it progresses, which eventually leads to death. It is believed that some plaques and tangles develop within the structure of the brain which causes brain cells to die. Alzheimer’s patients also have a deficiency of neurotransmitters which ultimately hampers the transmission of messages in the brain. It was confirmed by Amyloid hypothesis that beta-amyloid (βA) deposits are the fundamental cause of the disease. So, in this research natural and synthetic compounds were selected on the basis of their binding or inhibition to the amyloid precursor protein (APP). The protein and the ligands were optimized, docked and their interaction was visualized on the basis of binding energy.