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Sharan, Abhishek
- Comparative Analysis of Antibacterial and Antioxidant Activity of Coriandrum sativum, Mentha pipereta and Spinacia oleracea
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Authors
Affiliations
1 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., IN
1 Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agricutlure, Technology and Sciences, Allahabad, U.P., IN
Source
Asian Journal of Bio Science, Vol 8, No 2 (2013), Pagination: 205-208Abstract
The present study was carried out to evaluate the antibacterial and antioxidant activity of the crude ethanolic an acetone extracts of Mentha pipereta, Spinacia oleracea and Coriandrum sativum. The agar well diffusion assay and MIC test were carried out against four strains of bacterial species, viz., Staphylococcus aureus, E. coli, Bacillus pumulis, and Klebsiella pneumonia to determine the sample's antibacterial activity. The extracts of the plants at a concentration of 50 ?l/disc (200 mg/ml) showed minimum to moderate activity against bacteria indicating a broad spectrum activity. Variable concentrations of ethanolic extract of plant samples were effective against various pathogenic bacteria in MIC test. The result indicated the potential usefulness of these plants especially, in treating bacterial infections and justified the need for further investigations and characterization of the bioactive compounds present in the ethanolic and acetone extracts of the plants. On comparing the three plant materials, the chlorophyll content (both Chl A and Chl B) was found to be maximum in Spinacia oleracea leaves. The carotene content was found to be maximum in acetone extract of Mentha pipereta leaves, while minimum in Coriandrum sativum. Recently, attention has focused on phytochemicals as new sources of natural antioxidants. Therefore, the ethanolic crude extracts of the plant samples were screened for total phenols, flavonoids, and free radical scavenging activity. Free radical scavenging activity was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Significant differences in DPPH scavenging activity were found between the species investigated, ranging from 12.71 per cent to 68 per cent. The highest radical scavenging activity was observed in Coriandrum sativum (68% inhibition), followed by Mentha pipereta (61.62%) and Spinacia oleracea (54.72%). The total phenol content of the investigated species ranged from 26 to 75 mg CE/g extract, while flavonoids content ranged from 22 to 24 mg CE/g extract. The findings indicated promising antioxidant activity of crude extracts of the above plants and needs further exploration for their effective use in both modern and traditional system of medicines.Keywords
Mentha Pipereta, Spinacia Oleracea, Coriandrum Sativum, Staphylococcus Aureus, E. Coli, Bacillus Pumulis, Klebsiella Pneumonie, Flavonoids
References
- Aruoma, O.I. (1998). Free radicals, oxidative stress and antioxidants in human health and diseases. J. American Oil Chem.Soc., 75(2): 199-212.
- Baris, O., Golloce, M., Sahin, R., Ozer, H., Killic, H. and Ozkan, H. (2006). Biological activities of essential oil and methanol extract of Achillea bickersteinii. (Asteraceae). Turkish J. Biol., 30: 65-73.
- Bray, H.G. and Thorpe, W.V. (1954). Biochemical estimation for phenolic compounds. J. Antimicrobiol. & Chemotherapy., 1: 27-52.
- Buhler, D.R., Miranda, C.L., Stevens, J.F., Ivanov, V., Mark McCall., BalzFrei. and Deinzer, M.L. (2000).Antioxidant and pro-oxidant actions of prenylated and nonprenylated chalcones and flavanones in vitro. J. Agric. & Food Chem., 48(9): 3876–3884.
- Cai, Y., Luo, Q., Sun, M. and Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci., 74: 2157-2184.
- Chan, E.W.C., Lim, Y.Y. and Chew, Y.L. (2007). Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. Food Chem., 102(4): 1214-1222.
- Hanen, F., Hafedh, H., Najla, T., Emira, N., Mejdi, S., Riadh, K. and Amina, B. (199 B8)i.ological activities of the essential oils and methanol extract of tow cultivated mint species (Mentha longifolia and Mentha pulegium) used in the Tunisian folkloric medicine. World J. Microbiol. & Biotechnol., 25: 2227-2238.
- Kubo, I., Fujita, K., Kubo, A., Nihei, K. and Ogura, T. (2004). Antibacterial activity of coriander volatile compounds against Salmonella choleraesuis. J. Agric. & Food Chem., 52(11): 3329-3332.
- McClements, J. and Decker, E.A. (2000). Lipid oxidation in oil-water emulsions: Impact of molecular environment or chemical reactions in heterogeneous food system. J. Food Sci., 65(8): 1270-1282.
- Miliauskas, G., Venskutonis, P.R. and Beek, T.A. (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem., 85: 231-237.
- Morena, E., Mishra, R.P., Arshad, M. and Sami, A. (2000). Antibacterial properties of Rosa indica (L.) stem, leaves and flowers. Electronic J. Biol., 12(15): 4564-4573.
- Pascal, J., Delaquis, K., Stanich, B. and Girard, M.G. (2001). Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Agric. & Agri-Food Canada, Pacific Agri-Food Res. Centre., 23: 34-35.
- Pitchaon, M., Suttajit, M. and Pongsawatmani, R. (2007). Assessment of phenolic content and freeradical scavenging capacity of some Thai indigenous plants. Food Chem., 100: 1409-1418.
- Pokorny, J., Yanishlieva, N. and Gordon, M. (2001). Antioxidants in food. Practical applications. Cambridge Woodhead Publishing Limited., 72 (5): 145-171.
- Saleem, A., Ahotupa, M. and Pihlaja, K. (2000). Total phenolic concentration and antioxidant potential of extracts of medicinal plants of Pakistan. Zeitschrift fur Nature forschung., 56: 973-978.
- Saric, M., Kastrori, R., Curie, R., Cupina, T. and Gerie, I. (1976). Chlorophyll determination. Univ. Unoven Sadu Parktikum is fiziologize Bibjoke, Beagard, Hauncna, Anjig. 215. Curr. Sci., 5: 23-25.
- Uma, B., Prabhakar, K., Rajendran, S. and Sarayu, Y.L. (2000). Antimicrobial activity and phytochemical analysis of Coriander sativum against infectious diarrhoea. Ethnobotanical Leaflets., 5(4):12-14.
- Young, I.S. and Woodside, J.V. (2001). Antioxidants in health and disease. J. Clin. Pathol., 5: 176–186.
- Zheng,W. and Wang, S.Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. J. Agric. & Food Chem., 49(11): 5165-5170.
- Citric Acid Production from Pre-Treated Sugarcane Bagasse by Aspergillus niger under Solid State Fermentation
Abstract Views :370 |
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Authors
Affiliations
1 Department of Biochemistry and Biochemical Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
1 Department of Biochemistry and Biochemical Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
Source
Asian Journal of Bio Science, Vol 10, No 2 (2015), Pagination: 162-166Abstract
Citric acid is one of the important commercially produced organic acid. The aim of the present work was to study the utilization of pre-treated sugar cane bagasse for citric acid production using Aspergillus niger under solid state fermentation. The maximum value of citric acid was observed in acid treated substrate followed by urea and heat respectively. Fermentation conditions were also optimized and maximum production of citric acid occurred when the pH, Initial moisture content and temperature of the fermentation medium were 5, 65 per cent and 30°C, respectively. As a globally required organic acid for various industrial applications, citric acid can be produced at large scale by utilizing pre-treated agro residues such as sugarcane bagasse. Application of agro residues in the production of value added product can be a positive step towards agricultural waste management.Keywords
Citric Acid, Aspergillus niger, Pre-Treatment, Solid State Fermentation.References
- Ali, S., Haq, I.U., Qadeer, M.A. and Iqbal, J. (2002). Production of Citric acid byAspergillus niger using cane molasses in a stirred fermenter. Electronic J. Biotechnol., 5 (3) : 125-130.
- AOAC (1995). Official methods of analysis. 16th Ed. Association of Official Analytical Chemists, Washington D.C.
- Boominathan, S., Dhanaraj, T.S. and Murugaiah, K. (2012). Production and optimization of citric acid byAspergillus niger using pineapple and tapioca waste. Herbal Tech Industry, 12 : 8-11.
- Heinzle, E., Biwer, A.P. and Cooney, C.L. (2007). Development of sustainable bioprocess: Modeling and assessment. John Wiley and Sons, England.
- Kareem, S.O., Akpan, I. and Alebiowu, O.O. (2010). Production of Citric acid byAspergillus niger using Pineapple waste.Malay J. Microbiol., 6 (2) : 161-165.
- Khosravi, D.K., Zoghi, A., Alavi, S.A. and Fatemi, S.S.A. (2008). Application of Plackett Burman Design for Citric acid production from pretreated and untreated wheat straw. Iran J. Chemical Engg., 27(1) : 91-104.
- Kim, S.K., Park, P.J. and Byun, H.G. (2002). Continuous production of Citric acid from dairy wastewater using immobilized Aspergillus niger ATCC 9142. Biotechnol. & Bioprocess Engg., 7: 89-94.
- Kumar, D., Jain, V.K., Shanker, G. and Srivastava, A. (2003). Utilization of fruits waste for citric acid production by solid state fermentation. Process Biochem., 38 : 1731-1738.
- Lee, Y.Y., Lyer, P. and Torget, R.W. (1999). Dilute acid hydrolysis of Lignocellulosic biomass. Adv. Biochemical Engg. & Biotechnol., 65 : 93-97.
- Lofty, W.A., Ghanem, K.M. and El-Helow, E.R. (2007). Citric acid production by a novelAspergillus niger Isolate. II. Optimization of Process Parameters through Statistical Experimental Designs. Bioresource Technol., 98 : 3470-3477.
- Lu, M., Brook, J.D. and Madox, I.S. (1997). Citric acid production by solid state fermentation in a packed-bed reactor using Aspergillus niger. Enzyme Microbiol. Technol., 21 : 392-397.
- Narayanamurthy, G., Ramachandra, Y.L., Rai, S.P., Manohara, Y.N. and Kavitha, B.T. (2008). Erica husk: An inexpensive substrate for citric acid production by Aspergillus niger under solid state fermentation. Indian J. Biotechnol., 7 : 99-102.
- Nwoba, E.G., Ogbonna, J.C., Ominyi, M.C., Nwagu, K.E. and Gibson, U.G. (2012). Isolation of Citric acid producing fungi and optimization of Citric acid production by selected isolates. Global J. Biosci. & Biotechnol., 1(2) : 261-270.
- Soccol, C.R., Vandenberghe, L.P.S., Rodrigues, C. and Pandey, A. (2006). New perspectives for Citric acid production and application. Food Technol. & Biotechnol., 44 (2) : 141–149.
- Vandenberghe, L.P.S., Soccol, C.R., Pandey, A. and Lebeeult, J.M. (2000). Solid state fermentation for the synthesis of citric acid by Aspergillus niger. Bioresource Technol., 74 (2) : 175-178.
- Zar, J.H. (1984). Biostatistical analysis, Englewood cliffs NJ: Prentic hall Inc. 437-467.
- Comparative Study of Secondary Metabolites from Different Medicinal Plants
Abstract Views :183 |
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Authors
Affiliations
1 College of Biotechnology and Allied Sciences, Allahabad Agricultural Institute-Deemed University, Allahabad (U.P.), IN
1 College of Biotechnology and Allied Sciences, Allahabad Agricultural Institute-Deemed University, Allahabad (U.P.), IN