Asian Journal of Bio Science

S. P. Hadke ¹, A. G. Deshmukh ², M. S. Dudhare ², E. R. Vaidya ²

Affiliations
1 Department of Botany, Agnihotri College of Science, Wardha (M.S.), IN
2 Biotechnology Centre, Department of Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN

Abstract

Simarouba glauca is an oil yielding tree with a very high productivity and its rapid multiplication is essential for mitigating the edible/industrial oil demand. For developing in vitro regeneration protocol, optimization of surface sterilization of explant and study of effect of auxins and cytokinins is essential. The explant was exposed to mercuric chloride (0.1 %) and Sodium hypochloride (1 %) for a period of 2-12 minutes. Both the surface sterilizing agents gave near about same results with no contamination even at lowest contact time, however, as the contact time was increased intense browning was observed. The callus induction was observed at all the concentration of 2,4-D and BAP. More callusing was observed with higher concentration of both 2,4 D and BAP.

Keywords

Simarouba glauca, Callus, Auxins, Cytokinins.

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A. G. Deshmukh ¹

Affiliations
1 Biotechnology Centre, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN

Abstract

The use of immunoassays for analysis of small molecules has been extensive in endocrinology, clinical chemistry and other fields. However, small molecules such as pesticides are not able to induce immunogenic response by themselves but can generate immune response when conjugated to higher molecular weight compounds such as proteins. When the haptens are coupled to proteins the estimation of per cent conjugation is important as higher the concentration of hapten to protein more can be the specificity of developed antibodies. Here 2,4,6, trinitrobenzene sulphonic acid method was used to determine the per cent conjugation. Cypermethric acid, a metabolite of cypermethrin was used as hapten and was coupled to BSA and tetanus toxoid and the per cent conjugation was found to be 40% and 27 %, respectively.

Keywords

Cypermethric Acid, Hapten, Tetanus Toxoid, Per Cent Conjugation.

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M. S. Dudhare ¹, A. G. Deshmukh ²

Affiliations
1 Department of Biotechnology, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), IN
2 Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), IN

Abstract

Food crises in the sixties led to the development of high yielding varieties of cereals particularly, rice and wheat which resulted into green revolution and food production was increased significantly. A rapidly expanding population is a serious concern for food security especially for developing countries including that of India. To keep pace with the present population growth and consumption pattern, India’s food requirement has been estimated to 225 million tons by the year 2005. Green revolution technologies, though contributing to food production, they are inadequate to meet the challenges lie ahead. Recently developed biotechnological techniques undoubtedly have major role to play in solving the food security problem, just as the green revolution had its origins in science and technology, and particularly in science of genetics. So the application of novel biotechnological tools could lead to gene revolution. With the wide spread of advanced cellular and gene technology, the new technologies will certainly help in enhancing the crop productivity.

Keywords

Somaclones, Transgenic, Gene Technology, Food Crises, Productivity.

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M. S. Dudhare ¹, A. G. Deshmukh ¹

Affiliations
1 Department of Agricultural Botany, Dr. Panjabrao Deshmukh Agricultural University, Akola (M.S.), IN

Abstract

In Lactic streptococci several metabolic properties which are vital for successful dairy fermentation are unstable. With the advent of techniques for studying genetic composition of dairy streptococci, it has become possible to provide explanation for this unstable phenomenon. These organisms characteristically harbor many plasmid species (AkCelik, 1999). The number observed ranges from two to eleven, but most strains appear to contain four to seven distinct plasmid species.

Keywords

Subculturing, Metabolic, Activities, Srains Lactococcus Lactic.

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