Asian Journal of Bio Science

Open Access Open Access  Restricted Access Subscription Access
Open Access Open Access Open Access  Restricted Access Restricted Access Subscription Access

Isolation, Partial Purification, Product Formation and Characterization of β- Glucosidase from Roots of Hordeum vulgare L.


Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
     

   Subscribe/Renew Journal


β-glucosidase (EC 3.2.1.21) was extracted from ischolar_mains of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 17-54KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from ischolar_mains of Hordeum vulgare.


Keywords

Hordeum vulgare, β-Glucosidase, Purification, Characterization.
Subscription Login to verify subscription
User
Notifications
Font Size


Abstract Views: 181

PDF Views: 7




  • Isolation, Partial Purification, Product Formation and Characterization of β- Glucosidase from Roots of Hordeum vulgare L.

Abstract Views: 181  |  PDF Views: 7

Authors

Suman Tiwari
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
O. P. Verma
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India
Shruti Shukla
Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bio-Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), India

Abstract


β-glucosidase (EC 3.2.1.21) was extracted from ischolar_mains of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 17-54KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from ischolar_mains of Hordeum vulgare.


Keywords


Hordeum vulgare, β-Glucosidase, Purification, Characterization.