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Rhizogenesis in Cell Suspension Culture from Mango Ginger: A Source of Isosorbide and n-Hexadecanoic Acid


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1 Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu 620020, India
 

Curcuma amada Roxb. belongs to the monocotyledonous family Zingiberaceae. It is commonly known as mango ginger and used as a spice and valuable medicine. In this study, adventitious roots of C. amada have been successfully established from cell suspension culture. The highest percentage of adventitious root production was obtained from friable call us derived cell suspension culture. The culture conditions of adventitious root were optimized and the maximum adventitious root production was obtained in half strength MS liquid medium containing 0.3mg L-1 IBA along with 3% of sucrose after 5 weeks of culture. Among the different initial inoculum density, the best culture condition for root growth occurred at 10 g FWof initial inoculum density. GC-MS analysis revealed that the in vitro raised adventitious roots containing two valuable bioactive compounds, isosorbide and n-hexadecanoic acid. The outcome of the present work will be helpful for the large scale cultivation of adventitious roots for the production of valuable bioactive compounds.
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  • Rhizogenesis in Cell Suspension Culture from Mango Ginger: A Source of Isosorbide and n-Hexadecanoic Acid

Abstract Views: 293  |  PDF Views: 28

Authors

Chellappan Soundar Raju
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu 620020, India
Kandhan Varutharaju
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu 620020, India
Chandrasekaran Thilip
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu 620020, India
Abubakker Aslam
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu 620020, India
Appakan Shajahan
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, Tamil Nadu 620020, India

Abstract


Curcuma amada Roxb. belongs to the monocotyledonous family Zingiberaceae. It is commonly known as mango ginger and used as a spice and valuable medicine. In this study, adventitious roots of C. amada have been successfully established from cell suspension culture. The highest percentage of adventitious root production was obtained from friable call us derived cell suspension culture. The culture conditions of adventitious root were optimized and the maximum adventitious root production was obtained in half strength MS liquid medium containing 0.3mg L-1 IBA along with 3% of sucrose after 5 weeks of culture. Among the different initial inoculum density, the best culture condition for root growth occurred at 10 g FWof initial inoculum density. GC-MS analysis revealed that the in vitro raised adventitious roots containing two valuable bioactive compounds, isosorbide and n-hexadecanoic acid. The outcome of the present work will be helpful for the large scale cultivation of adventitious roots for the production of valuable bioactive compounds.