Asian Journal of Pharmaceutical Research and Health Care

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A rapid, selective and sensitive high performance liquid chromatographic method for the estimation of Penciclovir in human plasma has been developed. Chromatography was carried out on a Hypersil BDS C18 column using a mixture of 20mM Potassium dihydrogen phosphate buffer (pH 3.5 ± 0.05) and methanol (95:5 v/v) as the mobile phase. The eluents were monitored for the drug by UV detection at 254 nm. Acyclovir was used as an internal standard for this study. The retention times for Penciclovir and Acyclovir were found to be 6.2 and 9.2 min respectively. The method was found to be linear in the concentration range of 50.1 ng/mL to 7000.9 ng/mL. The method was validated as per FDA guidelines and was found to be suitable for bioequivalence and pharmacokinetic studies.

Keywords

Penciclovir, Determination, Plasma, HPLC.
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