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Properties of Bacillus species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate


Affiliations
1 Department of Biotechnology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
2 Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
3 Department of Food Technology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
 

The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost; cost of finished products and increases gross domestic products.

Keywords

Cellulase, Bacillus species, Crude Activities, Temperature, pH Tolerance, Brewers Spent Grains (BSG).
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  • Properties of Bacillus species Cellulase Produced Using Cellulose from Brewers Spent Grain (BSG) as Substrate

Abstract Views: 185  |  PDF Views: 22

Authors

Frank Anayo Orji
Department of Biotechnology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Ekaette Nduka Dike
Department of Biotechnology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Adekunle Kolawole Lawal
Department of Biotechnology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Abdulateef Omoniyi Sadiq
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Yewande Suberu
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Ariyo Caleb Famotemi
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Agatha Isioma Ugbana
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Folake Fashola
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Blossom Ita
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Samuel Olakiitan Olatope
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Emoleila Ejiya Itoandoan
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Abimbola Olajumoke Adefiranye
Department of Project Designs and Development, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria
Gloria Nwakaegho Elemo
Department of Food Technology, Federal Institute of Industrial Research, Oshodi (FIIRO), Ikeja, Nigeria

Abstract


The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost; cost of finished products and increases gross domestic products.

Keywords


Cellulase, Bacillus species, Crude Activities, Temperature, pH Tolerance, Brewers Spent Grains (BSG).